中文摘要：

从鲍鱼内脏中提取活性多糖,为提高鲍鱼内脏的附加值,促进鲍鱼的综合利用和开发提供理论和技术支持。鲍鱼内脏经碱性蛋白酶和胃蛋白酶处理后,用Sephadex G-100凝胶柱过滤层析分离出两个多糖组分,AVPⅠ和AVPⅡ,其多糖质量分数分别为66.41%和51.84%;蛋白质量分数为1.70%和15.55%;分子质量为272和6 ku;它们具有相同的单糖组成,但各种单糖的相对含量不同,AVPⅠ中鼠李糖、葡萄糖、木糖、甘露糖、半乳糖、岩藻糖的摩尔比为1.00∶2.15∶4.00∶5.36∶33.18∶45.14,而AVPⅡ中上述多糖的摩尔比则为1.00∶1.46∶1.33∶4.98∶16.08∶12.51;体外抗肿瘤结果显示,AVPⅠ和AVPⅡ对Hela细胞和K562细胞具有一定的抑制作用;体外免疫检测发现,AVPⅠ和AVPⅡ能够增强淋巴细胞增殖、腹腔巨噬细胞吞噬功能和NK细胞的杀伤能力。研究结果表明,鲍鱼内脏多糖具有抗肿瘤和免疫调节功能,可用于免疫增强性功能食品的开发。

英文摘要：

The polysaccharides AVP Ⅰ and AVPⅡ are extracted from the viscera of Haliotis Discus Hannai Ino and separated on Sephadex G-100 column. The polysaccharide contents of AVP Ⅰ and AVPⅡ are 66.41% and 51.84%, and the protein contents are 1.70% and 15.55% respectively. The molecular masses of AVP Ⅰ and AVP Ⅱ are 272 and 6 ku. AVP Ⅰ and AVP Ⅱ contain the same monosaccharide including rhamnose, glucose, xylose, mannose, galactose and fucose, the ratio of which for AVP Ⅰ is 1.00： 2.15 ： 4.00 ： 5.36 ： 33.18 ： 45.14, whereas AVPⅡ is 1.00 ： 1.46 ： 1.33 ： 4.98 ： 16.08 ：12.51. AVP Ⅰ and AVP Ⅱ could restrain the proliferation of Hela and K562 cell in vitro. AVP Ⅰ and AVPⅡ can dose-dependently induce a significant increase in lymphocyte proliferation, phagocytosis of macrophage and natural killer cell activity in vitro. The abalone viscera can be used potentially as an immuneomodulating agent.