中文摘要：

目的探讨在高糖作用下,体外培养的小鼠足细胞凋亡及内质网应激凋亡因子Caspase-12 m RNA和蛋白表达水平的变化及左归降糖益肾方含药血浆的调控作用。方法将体外培养的小鼠足细胞随机分为空白组（A组,25 mmol/L葡萄糖,10%空白血浆）、高糖组（B组,200 mmol/L葡萄糖,10%空白血浆）、左归降糖益肾方含药血浆组（C组,200 mmol/L葡萄糖,10%含药血浆）,4-苯基丁酸含药血浆组（D组,200 mmol/L葡萄糖,10%含药血浆）。培养48 h后,采用免疫荧光细胞化学法、流式细胞术检测足细胞凋亡情况;Western blot法、RT-PCR法分别检测Caspase-12蛋白或m RNA表达水平的变化。结果与A组比较：在高糖状态下B组足细胞凋亡率、Caspase-12 m RNA和蛋白的表达水平均升高,差异有统计学意义（P〈0.05）。与B组比较：C组、D组足细胞凋亡率,Caspase-12 m RNA和蛋白的表达水平均降低,差异均有统计学意义（P〈0.05）。与C组比较：D组足细胞凋亡率降低,差异有统计学意义（P〈0.05）;但Caspase-12 m RNA和蛋白的表达水平,差异均无统计学意义（P〉0.05）。结论 Caspase-12表达上调,是足细胞凋亡的分子机制之一;10%左归降糖益肾方含药血浆可能通过降低Caspase-12的表达水平,从而保护足细胞。

英文摘要：

Objective To investigate the level of protein and m RNA expressions of Caspase-12 and cell apoptosis in podocyte cells cultured in high glucose condition in vitro, and the effects of Zuogui Jiangtang Yishen decoction plasma on Caspase-12 expression in podocyte cells. Methods The cultured podocyte cells were divided into the control group（group A,25 mmo L/L glucose, 10% blank plasma）, high glucose group（group B, 200 mmo L/L glucose, 200 mmo L/L glucose, 10% blank plasma）, Zuogui Jiangtang Yishen decoction plasma group（group C, 200 mmo L/L glucose, 10% containing medicine plasma）,and 4-Phenylbutyric acid group（group D, 200 mmo L/L glucose, 10% containing medicine plasma）. After incubation for 48 h,the cell apoptosis were observed by flow cytometry and immunofluorescence stainings. The protein and m RNA expressions of Caspase-12 were measured by western blot and RT-PCR, respectively. Results Compared with group A, the cell apoptosis rate, expressions of caspase-12 protein and m RNA were increased, the difference were statistically significant（P〈0.05）.Compared with group B, the cell apoptosis rate and Caspase-12 expressions were decreased, the difference were statistically significant（P〈0.05）. Compared with group C, the cell apoptosis rate in group D was significantly decreased（P〈0.05）, but the expressions of Caspase-12 protein or m RNA were not statistically significant（P〉0.05）. Conclusion The up-regulated expression of Caspase-12 probably was one of the molecular mechanisms of podocyte apoptosis. 10% Zuogui Jiang Tang decoction plasma could repair the damaged podocyte by down-regulating Caspase-12 expressions.