中文摘要：

目的：研究聚酰胺树脂分离纯化芦荟蒽醌苷元的最佳工艺条件。方法：以芦荟蒽醌苷元的吸附量及洗脱率为指标，研究树脂吸附的最佳工艺条件、洗脱溶媒用量，并对树脂再生进行考察。结果：聚酰胺树脂对芦荟蒽醌苷元的适宜交换吸附条件：药液质量浓度0．22g·L^-1，流速为1．0mL·s^-1及pH5时，其动态饱和吸附量达到10．5mg·g^-1。然后以2倍体积10％乙醇、4倍体积80％乙醇为洗脱溶媒洗脱，芦荟蒽醌苷元洗脱率为94．5％。经过再生的树脂，吸附量相对稳定。结论：建立了一种蒽醌苷元的纯化工艺，为蒽醌苷元的工业化生产提供依据。通过高效液相色谱测定芦荟大黄素纯化前后的变化，证明该方法的有效性。

英文摘要：

OBJECTIVE To optimize the method of separation and purification of total anthraquinone aglycone in aloe using polyamide resin. METHODS Using the adsorption ratio and desorption rate of anthraquinone aglycone as the evaluating quo- tas, orthogonal experimental designs and variance analysis were applied to optimize the manipulation parameters of polyamide resin, meanwhile the resin regeneration was studied. RESULTS The optimal exchange adsorption condition of polyamide resin was as follows： the flow velocity was 1.0 mL· s^-1, solution concentration was 0. 22 g. L^-1 at pH 5.0, the dynamic saturated adsorption capacity reached to 10. 5 mg.g^- 1. After being eluted with twice as much 10% ethanol and quadruple as much 80% ethanol, the desorption rate was 94. 5%. CONCLUSION A technique for purification of anthraquinone aglycone was estab- lished, which provided the supporting for the industrialized production of aloe anthraquinone aglycone. The efficiency was demonstrated by analysis of aloe-emodin before and after purification using polyamide resin.