利用48对SSR引物分析了192份采集于江西49个县(区)的野生大豆遗传多样性。结果表明,共扩增出等位基因343个,平均每对引物扩增出7.2个等位基因,平均基因遗传多样性指数0.7369,平均多态性信息含量(PIC)0.7060,表明江西野生大豆具有较为丰富的遗传多样性。不同纬度和不同海拔的野生大豆其遗传多样性不同,高纬度及低海拔地区野生大豆遗传多样性要高。192份野生大豆可聚类成3大类,聚类结果与地理来源较为一致。
Using 80 SSR markers,192 wild soybean genotypes from Jiangxi province in China,were analyzed for genetic diversity.The results showed that total 343 alleles were identified,with 7.2 of the average of alleles per locus.The average genetic diversity index was 0.7369.The average polymorphism information content(PIC)was 0.7060.All these results indicated the abundance of genetic diversity among these genotypes.At the same time,genetic diversity were analyzed for genotypes from different latitude and altitude,which of circumpolar latitude and low altitude were higher.All the 192 wild soybean genotypes for molecular clustering could be clustered into 3 categories.It was found that the genetic distance was approximately consistent with the origin of the wild soybean genotypes.