中文摘要：

目的测定T、B淋巴细胞亚群在急性胰腺炎（acute pancreatitis，AP）患者中的水平，探讨其与AP病情的相关性。方法收集2013—04～2015—04重庆医科大学附属第一医院收治的AP患者200例作为病例组，并选择同期年龄、性别相近的健康体检者40例作为对照组。将AP患者分为轻症急性胰腺炎（mild acute pancreatitis，MAP）组（78例）、中重症急性胰腺炎（moderately severe acute pancreatitis，MSAP）组（73例）及重症急性胰腺炎（severe acute pancreatitis，SAP）组（49例）。应用流式细胞学技术分析T淋巴细胞亚群CD3^＋、CD4^＋、CD8^＋，CD4^＋CD25^＋调节性T细胞（regulatory T cell，Treg），以及调节性B淋巴细胞（regulatory B cell，Breg）的表达；应用酶联免疫吸附法（ELISA）测定IL-10和IL-4水平。结果MAP组、MSAP组及SAP组T淋巴细胞亚群CD3^＋、CD4^＋表达率及Breg表达率均低于健康对照组，且以SAP组及MSAP组下降更为明显，差异有统计学意义（P〈0．05）；MAP组、MSAP组及SAP组CD4^＋CD8^＋比值〈1．5（分别为41．0％、41．0％、63.3％）和比值〈1．0（分别为20．5％、31．5％、38．8％）的发生率均高于对照组（分别为22．5％和10．0％），差异有统计学意义（P〈0．05）。CD4^＋CD25^＋Treg表达率均明显升高，以SAP组升高最为明显（P〈0．05）。AP患者外周血CD3^＋、CD4^＋表达率及Breg与Ranson评分、APACHEⅡ评分及SOFA评分呈负相关性（P〈0．05）；AP患者CD4^＋CD25^＋Treg表达率与Ranson评分、APACHE1评分及SOFA评分呈正相关性（P〈0．05）。结论T、B淋巴细胞亚群改变与AP疾病病程和分级相关。

英文摘要：

Objective To detect the level of T and B lymphocyte subsets in patients with acute pancreatitis （AP）, and explore the correlation between the level of T and B lymphocyte subsets and the severity of AP. Methods Two hundred cases with acute pancreatitis were enrolled in the test group in the First Affiliated Hospital of Chongqing Medical University from April, 2013 to April, 2015, and 40 healthy volunteers in the control group. The test group was further divided into mild acute pancreatitis group （MAP, 78 cases）, moderately severe acute pancreatitis group （MSAP, 73 cases） and severe acute pancreatitis group （SAP, 49 cases）. T lymphocyte subsets including CD3^＋, CD4^＋, CD8^＋, CD4^＋ CD25^＋ regulatory T cell （Treg） and regulatory B cell （Breg） were measured using flow cytometry analysis. IL-10 and IL-4 level were measured using ELISA. Results Compared to the control group, there was a signif icant decrease in the percentage of CD3^＋, CD4^＋ T lymphocyte and Breg in MAP, MSAP and SAP group, especially in MSAP and SAP group （P 〈 0.05）. Compared to the control group, there was a significant increase of CD4^＋CD25^＋ Treg in MAP, MSAP and SAP group, especially in SAP group （P 〈 0.05）. There was a negative correlation between CD3^＋, CD4^＋ and Breg and the severity of acute pancreatitis （P 〈 0.05）, and there was a positive correlation between CD4^＋ CD25^＋ Treg and the severity of acute pancreatitis （P 〈 0.05）. Conclusion The level of T and B lymphocyte subsets was correlated with the severity of acute pancreatitis.