中文摘要：

目的研究肌醇加氧酶（myo-inositol oxygenase，Miox）基因在物种进化及爪蟾胚胎发育过程中的时间和空间表达的分布特点。方法利用半定量RT-PCR观察Miox在爪蟾胚胎发育过程中的时间表达模式，利用整体原位杂交方法观察Miox在爪蟾胚胎发育过程中的空间表达模式。结果RT-PCR结果显示Miox基因在胚胎发育第26期以前都没有表达，至胚胎发育第28期开始有微量表达，随着胚胎的发育其表达量逐渐增高；胚胎发育第40期表达明显升高，第41期时达到最高，第45期时表达有所下降。与胚胎发育第28、34期相比，第40、41、45期表达上调（P〈0．05）；与胚胎发育第40期相比，第41期表达上调（P〈0．05）；然而，同胚胎发育第41期相比，第45期表达下调（P〈0．05）。整体原位杂交方法发现在胚胎发育30期以前均没能检测到Miox在爪蟾任何器官中的表达，从33期开始，Miox在爪蟾前肾有很微弱的表达，且Miox的表达随着发育的进展逐渐升高。整体原位杂交方法结果同RT-PCR结果相类似，直至第39～40期，Miox的表达才明显升高，并且在随后的时期都以同样高的水平表达。另外，Miox基因在爪蟾胚胎发育过程中均仅仅在原肾小管表达。结论Miox是一个肾脏特异性基因，对于研究肾脏发育可能提供一个特异性标记。

英文摘要：

Objective To explore the molecular evolution of myo-inositol oxygenase （Miox） gene and its temporal and spatial expression patterns during the development of Xenopus laevis embryos. Methods The temporal and spatial expression patterns of Miox gene were analyzed by semi-quantitative RT-PCR and whole-mount in situ hybridization technique, respectively. Results RT-PCR results showed that Miox gene was hardly found before stage 26; slight expression was found at stage 28, which gradually increased thereafter, reaching a high level at stage 40 and peaked at stage 41; and then it had a decrease at stage 45. Compared with stages 28, 34, stages 40, 41, and 45 had a significantly higher Miox gene expression （P〈 0.05）. Compared with stage 40, stage 41 had a significantly higher Miox gene expression（P〈0. 05）. But stage 45 had a significantly lower expression compared with stage 41（P〈0.05）. The results of whole-mount in situ hybridization showed no Miox expression before stage 30; at stage 33 weak expression was found in the pronephros, and the expression gradually increased as time went by. The results of whole-mount in situ hybridization were consistent with that of RT-PCR, with Miox expression notably increased at stage 39-40, and then remained at that level. We also found that Miox was only expressed in the pronephros tubules during the whole embryo development period. Conclusion Miox is a kidney-specific gene during Xenopus laevis pronephros development, and it may serve as a marker for later pronephros development in organogenesis.