With the living standards gradually improved,the buildings with safe,comfortable,healthy indoor thermal environment would be the ideal pursuit. But to create the preferable indoor thermal environment,it is necessary to research physiological influence of indoor thermal environment on human body. So a typical region of hot-humid climate in Chongqing,China,was considered as the object to study physiological indexes of human body. And the indoor thermal environment parameters and physiological indexes of human body were the main measurements. 20 volunteer students were organized to take part in the experiments in the laboratory during this summer. And two methods,physical measuring and questionnaire investigation,were used in the experiments. The results show that the increase in indoor air temperature reduces the uncomfortable feeling of air draft. Indoor air temperature has visible effects on MCS (motor nerve conduction speed),SCS (sensory nerve conduction speed),HR (heart rate),the ECG (electrocardiogram)-QT segment and SSEP (short somatosensory evoked potential)-latent period of N9. Therefore,a safe,comfortable and healthful indoor environment can be created by considering these factors.