中文摘要：

[目的]探讨作为模式小麦中国春PBF转录因子编码基因的多样性。[方法]以中国春小麦品种为材料,利用基因特异引物进行PCR扩增,经过胶回收,将目标片段连接至克隆载体上,随机选择阳性质粒测序。序列拼接与比对、信号肽预测、二级结构预测、亚细胞定位及系统演化分析分别经由DNAMAN、Signalp、PSIPRED、Nuc_PLoc及MEGA软件进行分析比对。[结果]从六倍体普通小麦中国春基因组中克隆得到25条编码序列,根据序列相似性,将其聚成3种等位类型,亚基2个位点存在SNP,且都导致编码氨基酸残基的改变,并影响到最终编码产物的二级结构。[结论]PBF亚基编码序列在中国春小麦中极端保守,但仍然存在变异。该结果为评价小麦籽粒储藏蛋白亚基的表达效率提供了理论参考。

英文摘要：

[Objective] This study aimed to investigate the polymorphism of PBF en- coding genes from common wheat Chinese Spring （Triticum aestivum L.）. [Method] Using common wheat Chinese Spring as the experimental material, gene-specific primers were designed and applied to amplify the genomic DNA of Chinese Spring. PCR products were isolated, purified and ligated into the cloning vector. Positive clones were randomly selected for sequencing. A series of softwares including DNAMAN, Signalp, PSIPRED, Nuc_PLoc and MEGA were employed for sequence assembly and alignment, signal peptide prediction, primary and secondary structure prediction, as well as analyses of subcellular location and phylogenetic relationships between the PBF family members in Poaceae. [Result] Twenty-five target sequences were obtained from the genome of hexaploid common wheat Chinese Spring, which were classified into three clusters based on the sequence similarity. SNPs exist at two loci of the subunit, resulting in the change of encoded amino acid residues and affecting the secondary structure of final product encoded. [Conclusion] PBF encoding sequences are extremely conservative in Chinese Spring with certain variations. This study provides theoretical reference to evaluate the expression efficiency of wheat storage proteins.