中文摘要：

Trizol法从猪脾脏中提取总RNA,RT-PCR方法得到猪Akirin2的完整编码区,软件分析猪Akirin2的核酸序列与蛋白序列,进行染色体定位。将Akirin2构建到带有荧光标记的真核表达载体pEGFP-C1中,通过脂质体转染法将pEGFP-Akirin2转入猪脐静脉血管内皮细胞系（SUVEC）中进行表达。结果表明,该序列编码203个氨基酸,猪akirin2基因定位于猪1号染色体,含有4个外显子,猪与牛的Akirin2蛋白高度同源。重组表达质粒pEGFP-Akirin2经双酶切鉴定和测序分析,表明构建成功,pEGFP-Akirin2转染SUVEC细胞后,经荧光检测证实转入的akirin2基因得到表达。研究结果为探讨猪Aki-rin2蛋白在免疫调控中的功能提供了基础数据。

英文摘要：

We extracted whole RNA from porcine spleen using Trizol, and the intact ORF of porcine Akirin2 was obtained by RT-PCR. Using software to analyze its nucleic acids and protein sequences, we determine the chromosomal location of porcine Akirin2. Construction of eukaryotic expression vector was performed by amplifying akirin2 genes and then cloning it to pEGFP-C1 vector with fluorescence labeling. Finally, we transfected pEGFP-Akirin2 to porcine vascular endothelial cell line using liposome technique. Results show this gene encodes 203 amino acids, locates on porcine chromosome 1 and was highly homologous to that of bovine. After the transfection of pEGFP-Akirin2 into SUVEC, the eukaryotic expression was also validated by fluorescence analysis. The results provided the basic data for exploring the function of Akirin2 in the the immune regulation.