中文摘要：

在完整克隆出猪BCL10基因的基础上,用大引物PCR法构建3个真核表达载体：pEGFP-B（完整猪BCL10）载体、pEGFP-D（缺失CARD结构域）载体和pEGFP-P（缺失Thr-Ser-Ser位点）载体,转染猪血管内皮细胞（SU-VEC）,观察其表达情况,以此研究Thr-Ser-Ser位点（富含TS区）对猪BCL10募集功能的影响。结果显示,pEGFP-B仅在胞质中表达,并呈短棒状,而pEGFP-D与pEGFP-P则在胞质和核内均有表达。表明CARD结构域参与猪BCL10募集过程,且Thr-Ser-Ser为CARD结构域中关键氨基酸位点,这为进一步研究猪BCL10功能和作用机制奠定了基础。

英文摘要：

This study aimed at the impact of Thr-Ser-Ser site（T-S rich area） on swine BCL10 protein recruitment.After complete cloning of swine BCL10 gene,the vector was constructed using megaprimer PCR and transfected into swine umbilicus veins endothelial cell（SUVEC）.Three vectors were successfully constructed：pEGFP-B（complete swine BCL10）,pEGFP-D（without CARD domain） and pEGFP-P（without Thr-Ser-Ser site）.The results showed that pEGFP-D and pEGFP-P were expressed both in the cytoplasm and the nucleus of SUVEC transfected,and the complete swine BCL10 was only expressed like short rod in the cytoplasm of the cell.These results indicated that CARD domain was involved in swine BCL10 recruitment process and Thr-Ser-Ser was a significant site in the CARD domain,which provided a foundation for further research on the function and mechanism of swine BCL10.