中文摘要：

目的：探讨TLR4的激活对哮喘血清被动致敏人气道平滑肌细胞（HASMCs）增殖与合成分泌功能的影响。方法：通过培养原代HASMCs,以人哮喘血清致敏细胞,观察HASMCs表面TLR4的表达、细胞增殖反应与转化生子因子-β1（TGF-β1）在哮喘状态下的合成分泌水平。结果：与正常对照组相比,被动致敏组和TNF-α组HASMCsTLR4表达均显著增多（P〈0.01）,增殖反应显著增强（P〈0.01）,总蛋白浓度、IgE与TGF-β1的分泌水平均显著增高（P〈0.01）,并且TNF-α组各项指标均较被动致敏组效果更加显著;抗TLR4抗体组各项指标较被动致敏组、TNF-α组显著减弱或减少（P〈0.01）。结论：哮喘血清被动致敏HASMCs表面TLR4的活化可能通过诱导细胞的增殖和TGF-β1的合成分泌,导致哮喘气道微环境的改变,参与哮喘气道重构过程。

英文摘要：

Objective： To investigate the activation of Toll like receptor 4（TLR4） on passively sensitized human airway smooth muscle cells（HASMCs） proliferation and the synthesis and secretion function.Methods： Through the cultivation of primary HASMCs,we studied TLR4 expression on cell surface,cell proliferation and transformation of parturient factor-β1（TGF-β1） in asthma under the condition of synthesis and secretion level by passively sensitized HASMCs with asthma serum.Results： Compared with the control group,in passive sensitized group and TNF-α group TLR4 expression were significantly increased（P0.01）,significantly enhanced proliferation（P0.01）,total protein concentration,IgE secretion and TGF-β1 were significantly higher（P0.01）;and all the above parameters were increased more significantly in TNF group compared with those in the target effect of passively group;and those parameters were significantly reduced in anti-TLR4 antibody group compared with those in the target effect both of passively sensitized group and TNF-α group.Conclusion： TLR4 on passively sensitized HASMCs activated can induce the excessive proliferation of HASMCs and a large number of synthesis and secretion of TGF-β1,resulting in changing airway micro-environ-ment,which involved in airway remodeling in asthma.