中文摘要：

目的：建立茜草提取物中大叶茜草素的HPLC含量测定方法。方法：采用UltimateXB—C18（4．6mrnX250mm，5μm），流动相为甲醇-乙腈-0．2％磷酸（25：50：25），流速为1mL·min-1，柱温25℃，检测波长250rim。结果：在该条件下，大叶茜草素在3．566—456．4mg·L-1线性关系良好（r=0．9999）；平均回收率为98．97％（RSD2．29％）。结论：该方法准确、可靠，操作简便，可用于茜草提取物的质量控制方法。

英文摘要：

Objective： To establish a quantitative HPLC method for the determination of mollugin in extract from Rubia cordifolia L. Method： The determination was carried out on a Ultimate XB-C18 column at 25℃with methanol-acetonitrile-0. 2% phosphoric acid （25： 50： 25） as mobile phase and detection wavelength at 250 nm. The flow rate was 1.0 mL·min-l. Result： The good linearity was obtained with the correlation coefficients ranged of 3. 566-456.4 mg · L-1 （r =0.9999）. The average recovery rate was 98.97% （RSD 2.29%）. Conclusion： This method is accurate and reliable with easy operation, which can be used in the quality control of extracts from R. cordifolia.