中文摘要：

CDK11^p58属于CDK11/PITSLRE蛋白激酶家族成员,由Cdc2L2编码,是一种重要的细胞周期调控蛋白.为了研究CDK11^p58与胰腺癌细胞增殖的关系,我们通过采用脂质体转染真核表达载体及G418筛选的方式,获得了稳定过表达CDK11^p58的MIAPaCa-2（人胰腺导管腺癌细胞）单克隆细胞,并通过流式细胞分析、MTT检测及real-time PCR的方法检测了细胞周期、细胞增殖能力及G1/S期相关调控基因的转录水平.结果显示,该单克隆细胞（实验组）与空载体组细胞和空白对照组细胞相比G1期细胞比例明显下降（P〈0.01）,S期细胞比例明显上升（P〈0.01）;细胞增殖能力明显提高（P〈0.01）;cyclin D1、cyclin D3、p21基因mRNA水平较两组对照细胞明显升高（P〈0.01）.提示过表达的CDK11^p58通过上调cyclin D1、cyclin D3和p21基因的mRNA水平促进MIAPaCa-2细胞通过细胞增殖的关键限速点G1/S期,加快细胞增殖.

英文摘要：

CDK11^p58,a CDK11 /PITSLRE protein kinase family member,is encoded by Cdc2L2,and it plays a crucial role in cell cycle regulation.In order to investigate the relation between CDK11p58 and the proliferation of human pancreatic carcinoma cells,the recombinant eukaryotic expressional plasmid pcDNA3.0-HA-p58 was introduced into human pancreatic ductal adenocarcinoma cell line MIAPaCa-2 by Lipofectamine2000 and the cell was selected by G418.The cell clones which stably overexpress CDK11^p58 were identified by Western blot.Cell cycle,cell proliferation and G1 /S related gene mRNA levels were analyzed by flow cytometry assay,MTT assay and real-time PCR assay,respectively.Results showed that the S phase cell proportion,the proliferation activity and the mRNA level of cyclin D1,cyclin D3,p21 gene in pcDNA3.0-HA-p58 transfected cell were significantly increased compare with the cells in vector group and control group（P 〈0.01）.These results indicated that overexpression of CDK11^p58 could accelerate MIAPaCa-2 cell G1 /S progression and promote its proliferation.