中文摘要：

在现在的学习，我们检验了在 pseudorabies 之间的 codon 用法偏爱病毒(PRV ) US1 基因和 20 参考 alphaherpesviruses 的象 US1 一样基因。比较分析在 21 alphaherpesviruses 显示出同义的 codon 用法偏爱的显著不同，由 codon 改编索引， codons (ENc ) 的有效数字和 GC3s 价值显示了。PRV US1 基因的 codon 用法模式种系发生地被保存并且类似于 alphaherpesvirus 的类 Varicellovirus 的象 US1 一样基因的，与向有在第三个 codon 位置的 C 和 G 的 codons 的强壮的偏爱。有它的参考 alphaherpesviruses 的 PRV US1 基因的 codon 用法模式的簇分析证明 21 alphaherpesviruses 的象 US1 一样基因的 codon 用法偏爱与他们的基因功能有一种很靠近的关系。ENc 阴谋表明在 PRV US1 基因和 20 参考 alphaherpesviruses 的基因异质被 G+C 内容，以及基因长度抑制。另外，在 E 的有那些的 PRV 的 US1 基因的 codon 偏爱的比较。coli，酵母和人表明有 50 codons，显示出 PRV 和酵母之间的不同用法差别， 49 在 PRV 和人之间，但是 48 在 PRV 和 E 之间。coli。尽管有稍微在在 E.coli 和 PRV 之间的 codon 用法的更少差别，差别是不大可能的统计上重要、试验性研究是必要的为 PRV US1 建立最合适的表达式系统。在结论，这些结果可以改进我们进化，致病和 PRV 的功能的研究的理解，以及与另外的病毒贡献 herpesvirus 研究或甚至研究的区域。

英文摘要：

In the present study, we examined the codon usage bias between pseudorabies virus （PRV） US1 gene and the USl-like genes of 20 reference alphaherpesviruses. Comparative analysis showed noticeable disparities of the synonymous codon usage bias in the 21 alphaherpesviruses, indicated by codon adaptation index, effective number of codons （ENc） and GC3s value. The codon usage pattern of PRV US1 gene was phylogenetically conserved and similar to that of the USl-like genes of the genus Varicellovirus of alphaherpesvirus, with a strong bias towards the codons with C and G at the third codon position. Cluster analysis of codon usage pattern of PRV US1 gene with its reference alphaherpesviruses demonstrated that the codon usage bias of USl-like genes of 21 alphaherpesviruses had a very close relation with their gene functions. ENc-plot revealed that the genetic heterogeneity in PRV US1 gene and the 20 reference alphaherpesviruses was constrained by G＋C content, as well as the gene length. In addition, comparison of codon preferences in the US1 gene of PRV with those ofE. coli, yeast and human revealed that there were 50 codons showing distinct usage differences between PRV and yeast, 49 between PRV and human, but 48 between PRV and E. coil Although there were slightly fewer differences in codon usages between E.coli and PRV, the difference is unlikely to be statistically significant, and experimental studies are necessary to establish the most suitable expression system for PRV US1. In conclusion, these results may improve our understanding of the evolution, pathogenesis and functional studies of PRV, as well as contributing to the area of herpesvirus research or even studies with other viruses.