[目的]对鸡舍空气和周围基质(饲料、粪便、灰尘、土壤)中镰孢菌(Fusarium)的浓度和分布进行定量研究,为控制从业人员和禽类Fusarium感染及预警提供科学依据。[方法]采用6级Anderson空气微生物采样器和曝皿法采集空气中样本,采用无菌袋随机采取周围基质中样本,进行实验室培养、计数、纯化。[结果]共得到镰孢菌分离株717株,以灰尘中Fusarium的浓度最高(2.8×10^5-1.5×10^6cfu/g,平均为8.8×10^5cfu/g)。[结论]空气中的镰孢菌分布与土壤中和灰尘中镰孢菌相关性较大。
[Objective] The aim of this study is to quantitatively analyze the Fusarium concentration and distribution in the air and the surrounding substrates(feed,feces,dust,soil) in the chicken house to provide a scientific basis for the control and early warning of Fusarium infection in poultry and practitioner.[Method] Air samples collected by a 6-stage Anderson air microorganism sampler and exposed culture plates as well as substrate samples gathered by aseptic bags were cultured,counted and purified in the lab.[Result] 717 strains of Fusarium isolates were obtained and the dust had the highest Fusarium concentration among the samples(2.8×10^5-1.5×10^6 cfu/g with an average of 8.8×10^5 cfu/g).[Conclusion] The Fusarium distribution in the air was in high correlation with those in soil and dust.