中文摘要：

目的初步评估小鼠骨髓间充质干细胞（BMSC）复合组织工程支架复合体植入兔角膜板层的可行性，并探讨其对加固人工角膜与宿主界面减少其并发症的效果。方法实验研究。体外：将红色荧光蛋白标记的小鼠BMSC体外复合于细胞外基质（ECM）支架（去细胞猪关节软骨ECM组织工程支架），4周及8周在荧光显微镜下观察其细胞存活状况，选用甲苯胺蓝染色法观察支架的细胞分布，及扫描电镜观察支架孔隙结构和附着细胞的情况。体内：将小鼠BMSC．支架复合体植入8只兔眼的角膜板层口袋，对侧眼作为对照植入无细胞的支架材料，植入后分别于2、4、8周取材，光镜下观察其组织病理学特点（HE染色），荧光显微镜下观察其细胞存活状况，使用活体成像仪观察8周时角膜内细胞存活状态；记录所有实验动物的眼前节状态。结果扫描电镜观察ECM支架可见多孔隙结构，能够满足细胞及组织黏附及生长，甲苯胺蓝染色可见复合ECM后，支架可见细胞分布。免疫荧光显微镜下观察，红色荧光蛋白（RFP）标记后的小鼠BMSC体外复合ECM支架后，体外及角膜层间，细胞均呈增生性生长（最长观察时间8周）。光镜下观察，与左眼相比，术后2、4、8周角膜基质层细胞数量逐渐增加，可见浅基质层有少量单核细胞和小鼠BMSC，胶原略变稀疏，排列整齐，无新生血管生成，所有兔角膜上皮完整，基本维持单核柱状上皮细胞形态；ECM支架形态难以辨认，和胶原呈融合生长。活体成像仪观察到小鼠BMSC．支架复合体植入兔角膜板层后8N，小鼠BMSC细胞处于存活状态。所有兔眼植入细胞．支架后，整个术后炎症反应轻微，结膜充血不明显，无新生血管，植片周围角膜组织透明。1周后，炎症反应基本消失，角膜透明，可清晰看见基质层的支架，4周后支架变稀薄，8周时可观察到支?

英文摘要：

Objective To complete a preliminary evaluation of the feasibility of implanting the complex of mouse bone marrow mesenchymal stem cells （BMSC） and a tissue engineering scaffold into rabbit corneal lamellae, based on which a solution may be proposed to consolidate the keratoprosthesis and the recipient surface, and to reduce the risk of complications. Methods This experimental study was composed of two parts. （1） In vitro： some mouse BMSC were marked with red fluorescent proteins （RFP） and integrated with a decellularized pig articular cartilage extracellular matrix （ECM） scaffold. The cell survival was observed under a fluorescence microscope at 4 and 8 weeks. The cell distribution was examined by toluidine blue staining. The pore structure and the cell adhesion were observed under a scanning electron microscope. （2） in vivo： the complex of mouse BMSC and a decellularized scaffold was implanted into the lamellar cornea of 8 rabbit eyes with the fellow eyes as the controls. The eyes were sampled for observation using HE staining under a light microscope at 2, 4 and 8 weeks, respectively. The cell survival was examined under a fluorescence microscope, and the intracorneal cell survival at 8 weeks was observed using in vivo imaging. The conditions of ocular anterior segment of all the experimental animals were recorded. Results （1） Under the scanning electron microscope, the ECM scaffolds showed satisfactory porosity required for the adhesion and growth of cells and tissues, and the cell distribution over the cell-scaffold complex can be observed by toluidine blue staining. （2） Under the immunofluorescence microscope, cell proliferation was observed in vitro and in the interlamellar space （the maximum observation time was 8 weeks） after the RFP-marked mouse BMSC were integrated in vitro with ECM scaffolds. （3） Under the light microscope （HE staining）, the stromal ceils were detected to increase at each timepoint. A small number of monocytes and some mouse BMSC were observe