运用有性杂交方法,以强辣灌木辣椒Capsicum frutescens H101为母本(P1)、强辣C.chinense PI439487为父本(P2)进行种间杂交,获得了C.frutescens×C.chinense种间杂种。对种间杂种F125个表型性状进行了观察比较,结果表明:F1在生长势方面具有明显的杂种优势,其他表型性状则多介于P1与P2之间。SRAP分析显示,F1与P1、P2共有带220条,占总位点数的53.92%,与P1或P2共有带143条,占35.05%,F1特异带和特异缺失带12条,点2.95%。F1与P1遗传相似系数为0.749,大于其与P2的遗传相似系数(0.740),说明杂种在DNA水平上更趋向于母本。C.frutescens×C.chinense种间杂种的获得,为C.chinense香味和多花基因的转移及新材料创制奠定了基础。
With manual pollination,an interspecific hybrid was obtained from the cross between a pungent Capsicum frutescens accession H101(P1)and the pungent C.chinense accession PI439487(P2).25 phenotypic characters were observed and compared among P1,F1 and P2.The results indicated the growth vigor of F1 had heterosis significantly,and the majority of the other phenotypic characters were intermediate to that of either parent.SRAP analysis showed that the Fl hybrid contained 220 bands of two parents,accounting for 53.92% of the total loci,and the percentage of 143 bands holding by F1 and either parent reached to 35.05%.The number and the percentage of the specific loci in F1 were 12 and 2.95% respective1y.The genetic similarity coefficient between F1 and P1 was 0.749,greater than that between F1 and P2(0.740),suggesting the hybrid was more similar to the female parent at DNA level.The obtainment of the interspecific hybrid of C.frutescens×C.chinense afford a basis for the transfer of the fruit flavor gene and the multiple-flower gene in C.chinense and the formulation of new materials in pepper.