中文摘要：

目的检测并分析肝癌细胞对人成熟树突状细胞（m DCs）基因转录水平的影响。方法利用免疫磁珠法分选出新鲜人外周血单核细胞,在体外经重组GM-CSF、IL-4和TNF-α诱导,培养并分化为m DCs。与Bel7402肝癌细胞共培养的m DCs为共培养组,正常培养的m DCs为对照组。共培养48 h后,Trizol法抽提总RNA,利用人树突状细胞基因芯片检测m DCs的基因在转录水平的变化表达。结果差异表达的基因共有663个（与对照组相比,变化表达倍数≥2）,其中上调290个,下调373个。这些基因的功能主要涉及信号转导、细胞代谢和细胞周期等。结论肝癌细胞能在转录水平影响m DCs中多个基因的表达,这些基因控制并影响着m DCs的功能、增殖、分化和凋亡。

英文摘要：

Objective To detect the effects of hepatocellular carcinoma cells on the transcription of functional genes in maturate dendritic cells（ m DCs）. Methods Peripheral blood mononuclear cells（ PBMCs） were enriched from freshly harvested whole blood by immunomagnetic beads. MDCs were induced by GM-CSF,IL-4 and TNF-α in vitro. Then,m DCs were cultured with（ co-cultured group） or without（ control group） hepatoma cells. Forty-eight hours later,total RNA of m DCs was collected and the expressions of functional genes were analyzed by using human dendritic cells gene array. Results There were 663 genes significantly regulated（ compared with control group,fold changes≥ 2） in cocultured group,of which 290 were up-regulated and 373 were dow n-regulated. These genes were associated with the functions of signal transduction,cell metabolism,cell cycle,and so on. Conclusion Hepatocellular carcinoma cells affect the transcription of some functional genes in m DCs,which thus affects its functions,proliferation,differentiation and apoptosis.