中文摘要：

根据NCBI数据库中的黄曲霉细胞壁蛋白AFLMPl的基因序列设计特异引物，以黄曲霉菌总RNA逆转录获得的cDNA为模板，PCR扩增目的基因后分别克隆到pGEX-6p-1和pET-22b原核表达载体中，转化大肠杆菌BL21（DE3）感受态细胞。重组大肠杆菌经IPTG诱导表达后超声波破碎提取可溶性蛋白，SDS-PAGE电泳和Western杂交检测表达产物，结果表明GST-AFLMP1和AFLMP1-His融合蛋白能够在大肠杆菌中大量可溶性表达，可分别作为制备AFLMP1特异抗体的免疫抗原和筛选抗原。

英文摘要：

Specific primers were designed according to the gene sequence of the cell wall protein AFLMP1 of Aspergillus flavus that derived from the database of NCBI. Total RNA ofAspergillus flavus was extracted and reverse transcribed into cDNA to take as template. Then, A FLMP1 gene was amplified by PCR and respectively cloned into pGEX-6p-1 and pET-22b to construct prokaryotic expression vectors. Subsequently, the recombinant plasmids were transformed into Escherichia coli BL21 （DE3）, and then induced for protein expression by IPTG. The bacteria were treated using ultrasonic disruption and the soluble protein was extracted. After purification, the proteins were detected by SDS-PAGE and Western blotting. The results showed that both GST-AFLMP1 and AFLMP1-His fusion proteins could be largely expressed in bacteria with soluble form. They can be respectively used as immunogen and screening antigen for preparing AFLMP1 specific antibody.