中文摘要：

目的：探讨在原发性肝癌发展的不同阶段肝糖原的变化规律。方法清洁健康BALB／c雄性小鼠40只，分为正常组（ n =3）与模型组（ n =37）。将正常组3只小鼠处死，取肝；模型组小鼠每日饲以二乙基亚硝胺（ DEN ）溶液30μg· mL-1为饮用水，于诱癌4，8，12，16，20，24周，各取3只小鼠进行肝取材（ n=21）。将各组肝组织进行糖原过碘酸雪夫氏（ PAS）染色。并对样本进行平均光密度分析，用ANOVA显著分析和Student -Newman-Keuls检验。结果正常组和8，12周模型组的糖原含量依次显著降低，16周模型组糖原含量达最低；随后20，24周模型组肝糖原含量依次显著增加。结论 DEN诱导小鼠发生肝癌的过程中，肝糖原的变化规律为先减少后增加。

英文摘要：

Objective To analyse the change of hepatic glycogen in the process of hepatocellular carcinoma development in N -nitrosodiethy-lamine（DEN） -induced mice, and investigate the relationship between the hepatic glycogen content and the different stage of liver cancer . Methods There were forty clean and healthy male mice .They were di-vided in two groups ,the normal group （ n=3 ） and the model group （ n=37）.After adapting environment in one week ,the group of normal mice were sacrificed and all the model animals were administrated by solution of DEN （30 μg· mL-1 ） in the carcinoma-induced period.Every three mice were sacrificed in the this group induced by DEN in 4 , 8 , 12 , 16 , 20 ,24 weeks（n=21）.The obtained liver tissues divided in seven groups and embedded by paraffin were cut at thick piece .After staining with pe-riodic acid-Schiff （ PAS） the hepatic glycogen were observed .In order to obtain the mean optical density , samples of each group were disposed by the software Motic Images Advanced 3.2.Lastly.Analysis of variance （ ANOVA） test was followed by individual comparison by Student -New-man-Keul test using SPSS V16.0.Result The contents of hepatic gly-cogen in normal group , 8-week and 12-week groups were decreased successively and significantly , and the 16-week were least .The con-tents of hepatic glycogen in 16-week compared with 20-week and 24-week groups were increased successively and significantly .Conclusion In the process of development of hepatic carcinoma induced by DEN , the regular of hepatic glycogen′s change was decreasing gradually at the prior period ,and then the level of hepatic glycogen increased later .