中文摘要：

目的研究肿瘤坏死因子-α（TNF-α）在小鼠酒精性肝损伤过程中的表达与意义。方法将50只健康雄性小鼠按照体重随机分为2组：对照组（n=10）,模型组（n=40）。用56°红星二锅头经酒精灌胃（15 mL·kg^-1）诱导小鼠肝损伤1,2,3,4周,建立小鼠酒精性肝损伤模型,分别于0（对照组小鼠）与1,2,3,4周,各取10只小鼠于眼球取血,用赖式法检测血清谷草转氨酶（AST）的活性;以颈椎脱臼法处死小鼠,取小鼠肝,用蛋白印迹法测定肝组织TNF-α的表达量。结果小鼠肝AST酶的活性在0,1,2,3,4周分别为（112±22）,（126±24）,（967±30）,（1010±35）,（206±23）U·L^-1,与对照组相比,差异均有统计学意义（均P〈0.05）。模型组的小鼠肝AST酶的活性在第1周缓慢上升,第2周迅速上升,于第3周达到高峰,第4周开始下降,但仍高于对照组。TNF-α相对积分光密度在0,1,2,3,4周分别为1.15±0.12,1.10±0.08,2.13±0.16,2.16±0.15,1.16±0.11,与对照组相比,第1周略有下降,第2周迅速上升,第3周达到高峰,第4周出现下降,仍高于对照组,差异均有统计学意义（均P〈0.01）。结论在酒精灌胃诱导的小鼠肝损伤的进程早期,TNF-α促使肝细胞的损伤和坏死,并参与了后期肝的修复再生。

英文摘要：

Objective To investigate the dynamic changes and effects of tumor necrosis factor（ TNF-α） expression in mice with alcohol-induced liver injury. Methods According to the principle of random distribution,50 mice were classified into two groups,including the normal control group（ n = 40） and the model group（ n = 10）. Administered with alcohol of 56 degrees（ 15 mL·kg^-1） for 1 week and 2,3,4 weeks to induce liver injury. All mice in control group were executed to obtain the eye blood and the liver tissue in 0（ control group）,1 week and 2,3,4 weeks. The aspartate transaminase（ AST） activities in mice were detected by Reitman-Frankel methods. With the detection of Western blotting,the expressions of TNF-α were measured in the mice of control group and model group in the process of alcohol-induced liver injury.Results The activity of AST in liver of mice at 0（ control group）,1week and 2,3,4 weeks were（ 112 ± 22）,（ 126 ± 24）,（ 967 ± 30）,（ 1010 ± 35）,（ 206 ± 23） U·L^-1,respectively. Compared with control group,the differences were significantly（ all P〈0. 05）. Compared with thecontrol group,AST activities in the model group increased slowly in the first weeks,then rose rapidly in the second week,reached the peak in the third week,began to decline in the fourth week,but still higher than the control group. TNF-αlevel at 0,1 week and 2,3,4 weeks were 1. 15 ± 0. 12,1. 10 ± 0. 08,2. 13 ± 0. 16,2. 16 ± 0. 15,1. 16 ± 0. 11 respectively.Compared with the control group,the differences were significantly（ all P〈0. 01）. Compared with the control group,the expression of TNF-α in the model group gradually rose from the first week to the third week,then fell close to normal level across time. Conclusion The expression of TNF-α had obvious varieties in the process of alcoholic hepatic injury in mice,which demonstrates TNF-α may have significant impact on the injury and restoration of liver tissue.