中文摘要：

目的 观察温针的镇痛效应并探讨其与穴区肥大细胞的功能关系、穴位特异性。方法 SD大鼠60只,随机分为空白组（C）、模型组（M）、足三里温针组（Z）、对侧温针组（O）、手针组（A）、伏兔温针组（F）、阳陵泉温针组（Y）、委中温针组（W）、色甘酸钠（Disodium cromoglycate）＋温针组（DSCG＋Z）、生理盐水＋温针组（Saline＋Z）。以佐剂型关节炎大鼠为炎性反应痛模型（AA模型）,在体观察温针足三里、阳陵泉、伏兔、委中过程中大鼠的缩爪反射潜伏期;并通过穴位组织切片染色,离体对照治疗前后穴位处局部肥大细胞脱颗粒率的变化,以及穴区注射色甘酸钠对其的影响。结果 温针后穴区肥大细胞脱颗粒现象明显（P〈0.05）,温针镇痛的效果要优于手针治疗（P〈0.05）,足三里温针组经治疗后的痛阈均显著高于伏兔温针组、阳陵泉温针组和委中温针组（P〈0.05）,针刺引起的肥大细胞脱颗粒现象能被色甘酸钠显著阻断（P〈0.05）。结论 温针佐剂性关节炎大鼠足三里穴可产生镇痛效应,其镇痛效果优于手针组,且穴区处的肥大细胞脱颗粒率高于手针组,使用肥大细胞膜稳定剂色甘酸钠预处理后,温针的镇痛效应减弱。对于AA模型,温针足三里穴的镇痛效果优于阳陵泉、伏兔、委中穴。

英文摘要：

Objective To investigate the analgesic effect of warm needling and explore its relationship with the function of mast cells in the acupoint area and the specificity of acupoints. Method Sixty SD rats were randomized into blank （C）, model （M）, warm needling Zusanli （Z）, contralateral warm needling （O）, manual acupuncture （A）, warm needling Futu （F）, warm needling Yanglingquan （Y）, warm needling Weizhong （W）, disodium cromoglycate ＋ warm needling （DSCG＋Z） and saline ＋ warm needling （Saline＋Z） groups. A rat with adjuvant arthritis was used as a model of inflammatory pain （AA model）. Hindpaw withdrawal latency was measured in the rats during warm needling at Zusanli （ST36）, Yanglingquan （SP9）, Futu （ST32） and Weizhong （BL40）. Pre-treatment and post-treatment rates of mast cell degranulation in the acupoint area were compared and the effect of acupoint area injection of disodium cromoglycate on the degranulation was observed by acupoint tissue section staining. Result Obvious degranulation of mast cells in the acupoint area appeared after warm needling （P〉0.05）. The analgesic effect of warm needling was better than that of manual acupuncture （P〉0.05）. After treatment, pain threshold was significantly higher in the warm needling Zusanli group than in the warm needling Futu, warm needling Yanglingquan and warm needling Weizhong groups （P 〉 0.05）. Acupuncture-induced mast cell degranulation could be prevented by disodium cromoglycate （P〉0.05）. Conclusion Warm needling at point Zusanli can produce an analgesic effect in adjuvant arthritis rats. Its analgesic effect is better than that of manual acupuncture. The rate of mast cell degranulation in the acupoint area is higher in the warm needling Zusanli group than in the manual acupuncture group. The analgesic effect of warm needling is reduced after pretreatment with disodium cromoglycate, a mast cell stabilizer. In an AA model, the analgesic effect of warm needling at point Zusanli