中文摘要：

MIN6细胞给予400 μmol/L H2O2处理分为正常对照组、正常加H2 O2组、Scramble siRNA（Small interfering RNA）组和RIP140 siRNA组,400 μmol/L H2O2能增加MIN6细胞的凋亡率（27.89％±1.91％对3.74％±0.55％,P＜0.01）和丙二醛含量[（35.17±6.26对20.07±1.86） nmol/mg,P＜0.05],明显减少MIN6细胞活力（24和48 h,均P＜0.01）、总超氧化合物歧化酶水平[SOD,（4.79±0.85对3.09±0.60） U/mg,P＜0.05].下调受体相互作用蛋白140（RIP140）表达能减少H2O2诱导的MIN6细胞凋亡率（11.45％ ±2.27％对30.39％±5.32％,P＜0.01）和丙二醛含量[（22.32±1.94对33.42±2.51）nmol/mg,P＜0.01],增加总SOD水平[（5.32±0.40对3.09 ±0.15）nmol/mg,P＜0.01）].

英文摘要：

MIN6 cells cultured with 400 μmol/L H2O2 were divided into normal control group,control group with 400 μmol/L H2O2,scrambled siRNA group,and RIP140 siRNA group.400 μmol/L H2O2 could significantly increase apoptosis rate of MIN6 cells（27.89％ ± 1.91％ vs 3.74％ ±0.55％,P＜0.01） and the content of maleic dialdehyde [M DA,（35.17 ± 6.26 vs 20.07 ± 1.86） nmol/mg,P＜0.05],suppress cell viability （24 and 48 h,both P＜0.01）,and lower the level of total superoxide dismutase [SOD,（4.79 ± 0.85 vs 3.09 ± 0.60） U/mg,P＜ 0.05].When receptor interaction protein 140 （RIP140） expression was suppressed by RNA interference,the apoptosis rate of MIN6 cells （11.45 ％ ± 2.27％ vs 30.39％ ± 5.32％,P＜0.01） and the content of MDA [（22.32 ± 1.94 vs 33.42 ± 2.51） nmol/mg,P＜0.01] were reduced,and levels of total SOD was increased [（5.32 ± 0.40 vs 3.09±0.15） nmol/mg,P＜0.01）].