中文摘要：

目的观察过表达NR2E1能否保护MIN6细胞抵抗棕榈酸（palmitate,PA）所致的内质网应激凋亡,为糖尿病分子流行病学研究策略提供新的思路。方法建立过表达NR2E1小鼠胰岛细胞系MIN6细胞株,随后给予500μmol/L PA处理24h,利用流式细胞仪检测细胞凋亡率,实时定量PCR（RT-PCR）法检测CCAAT/增强子结合蛋白同源蛋白（C/EBP-homologous protein,CHOP）和重链结合蛋白（heavy-chain binding protein,BiP）mRNA的表达变化。结果过表达NR2E1能减少PA诱导的MIN6细胞凋亡率（P〈0.01）和CHOP mRNA表达（P〈0.01）,增加BiP mRNA表达（P〈0.05）。结论过表达NR2E1可以抵抗PA诱导的胰岛β细胞内质网应激凋亡,在分子水平为预防糖尿病发生发展提供了一些参考。

英文摘要：

Objective To investigate the effect of over-expression of NR2E1 on endoplasmic reticulum（ ER） stress and cell apoptosis. Methods Up-regulating NR2E1 expression before exposure to 0. 5m M palmitate for 24 h. Cell apoptosis was measured by using flow cytometry. The expression levels of NR2E1,CHOP,Bi P mRNA were detected by real-time RT-PCR. Results Upregulating NR2E1 declined cell apoptosis in palmitate-treated MIN6 cells（ P〈0. 01）. The expression levels of CHOP mRNA declined（ P〈0. 01）,and the expression levels of Bi P mRNA increased（ P〈0. 05）. Conclusion Up-regulation of NR2E1 expression may attenuate palmitate induced endoplasmic reticulum stress in pancreatic beta cells.