以吸水链霉菌(Streptomyces hygroscopicus)CCTCC M203062为出发菌株,采用N离子注入和紫外诱变处理,得到MTG酶活达到7.19U/ml的高产菌株BB-2。对BB-2再进行紫外诱变,选育出具有较好稳定遗传性的抗2-脱氧-D-葡萄糖的高产突变株DG-1,酶活达到9.21U/ml,比出发菌株提高了118.5%。
In the present study, through mutagenizing Streptomyces hygroscopicus CCTCC M203062 with UV and Nimplantation, a mutant strain BB-2 with TGase activity of 7.19 U/ml was obtained. Then a new stain DG-1 resistant to 2-deoxy- D-glucose (2-DG) repression was obtained from the mutant strain BB-2 by repeated UV radiation, with good genetic stability and TGase activity of 9.21 U/ml, which was higher 118.5% than that of the original strain CCTCC M203062.