中文摘要：

以6-甲氧基-β-（5-乙烯基-2-奎宁环基）-4-喹啉甲醇（奎尼丁，Qd）为单体合成了一种pH敏感荧光高分子聚N-异丙基丙烯酰胺（NIP）-奎尼丁-N，N，-二甲基氨丙基甲基丙烯酰胺（DMAPM）[P（NIP—DMAPM—Qd）]。采用共聚法将布氏杆菌抗原（BrAg）与P（NIP—DMAPM—Qd）连接，制备了P（NIP—DMAPM—Qd）-BrAg连接物。加入待测布氏杆菌抗体（BrAb），免疫反应后，调节至pH7．6，使高分子相变，从而将体系中的免疫反应复合物P（NIP—DMAPM—Qd—BrAg）-BrAb和未反应连接物P（NIP—DMAPM—Qd）-BrAg与非特异性组分以及未反应的待测BrAb分离．利用蛋白A对抗体的亲和性，捕获P（NIP—DMAPM—Qd—BrAg）-BrAb，从而将其与P（NIP-DMAPM—Qd—BrAg）分开，通过测定P（NIP-DMAPM—Qd—BrAg）-BrAb的荧光来定量BrAb。该方法通过高分子相变分离和蛋白A捕获双重分离作用，基本消除了非特异性组分以及未反应的特异性免疫成分等的干扰；以高分子自身荧光为检测信号源，无须另外的标记物，大大提高了免疫分析的灵敏度和简便性。以布氏杆菌抗体（BrAb）为检测对象，测得线性范围为0-168ng／L，抗体检出限为1．2．g／L，结果令人满意。

英文摘要：

A pH-induced phase transition fluorescent polymer was prepared by polymerization of N- isopropylacrylamide （NIP）, N-（3-dimethylaminopropyl）methacrylamide （DMAPM） and 6-Methoxy-β-（5-vinyl-2- quinicldinyl）-4-quinoline methanol （quinidine, Qd）. For the immobilization of Brucella melitensis antigen （BrAg）, the biocomposite-polymer conjugates P（NIP-DMAPM-Qd-BrAg） were obtained by introducing acryloyl-BrAg in the polymerization reaction of the aforementioned monomers, in the immunoassay procedure, the analyte BrAb reacted with P（NIP-DMAPM-Qd-BrAg） forming P（NIP-DMAPM-Qd-BrAg）-BrAb complexes, then the pH of solution was adjusted above the pH of the polymer （pHT.4） to precipitate the immunocomplex-polymer, and the complex precipitate was separated and re-dissolved by the adjustment of pH, finally the antibodies binding to the P （NIP-DMAPM-Qd-BrAg）-BrAb were absorbed by Staphylococcal protein A （SPA） embedded in sol-gel matrixes and quantified by measuring the fluorescence of the SpA-supported body surface. The calibration graph for BrAb was linear over the range of 0 -168 ng/L with a detection limit of 1.2 ng/L. The proposed method has been successfully used for BrAb analysis of the rabbit serum samples with satisfactory results.