利用傅里叶变换红外光谱、二阶导数谱首次对九州虫草不同部位进行了分析及评价研究。结果表明,九州虫草含有蛋白、多糖、核苷、脂类等活性成分,各部位的一维谱图相似度较高,人工子座与培养基之间的相似度高达0.9927,野生子座与虫体的差异则较为明显,二者相似系数为0.9499。二阶导数谱进一步丰富和确认了相应谱峰特征,证明了活性物质虫草素及腺苷的存在,提示了虫草多糖α-型和β-型糖苷键的存在。红外光谱图综合反映了虫草不同部位化学成分的多样性和复杂性,为虫草各部位的细微差异分析和评价及后期质控提供了快速、全面和客观的方法和手段,为九州虫草种质资源的开发利用提供了理论基础。
Fourier-transform infrared spectroscopy (FTIR) and second derivative spectra were used to analyze and evaluate the different parts of Cordyceps kyushuensis Kob in the present work. The results showed that C. kyushuensis contained proteins, polysaccharides, nucleosides, lipids and other active ingredients, the single-dimensional IR spectra of the various parts were highly similar, the similarity coefficient between the cultured stroma and medium reached up to 0. 992 7, and the natural stroma was more different from the parasites, with similarity coefficient of 0. 949 9. Second derivative spectrum further enriched and confirmed the feature of corresponding spectrum peaks, proved the existence of active substances such as eordycepin and adeno- sine, and prompted the presence of α and β-glycosidic bonds. The diversity and complexity of chemical constituents in different parts of C. kyushuensis were synthetically described by IR spectra, which provided a fast, comprehensive and objective approach to the analysis and evaluation of the imperceptible differences, and the quality control of Cordyceps. This work supplies a theo- retical basis for development and utilization of genetic resources C. kyushuensis.