目的:研究九州虫草体外不同极性部位的抗氧化作用。方法:采用DPPH法测定九州虫草石油醚(EVP)、乙酸乙酯(EVE)、正丁醇(EVB)和水(EVW)不同提取部位的抗氧化活性。结果:EVP,EVE,EVB,EVW均有DPPH自由基的清除能力,测得EVP,EVE,EVB和EVW清除DPPH的EC50值分别为190.2,141.4,151.6,195.4 mg·L-1。结论:九州虫草具有很强的抗氧化性,为进一步研究其抗氧化活性成分提供了科学依据。
Objective:To investigate the antioxidant effect in vitro of Cordyceps kyushuensis.Method:Antioxidant capacity of the petroleum ether fraction(EVP);acetic ether fraction(EVE);n-butanol fraction(EVB)and water fraction(EVW)were determined using the DPPH assay in vitro.Result:EVP,EVE,EVB and EVW showed antioxidant capacity of eliminating DPPH free radicals,and the scavenging efficiency increase with the concentration increase.EC50 values of the EVP,EVE,EVB and EVW are 190.2,141.4,151.6,195.4 mg·L-1 respectively.Conclusion:C.kyushuensis shows great oxidation resistance,and the results of the antioxidant effect of the EVP,EVE,EVB,EVW provide a scientific basis to the research of active constituent and the exploitation of C.kyushuensis.