中文摘要：

本研究以生长在3个不同温度（均温18．5℃，均温23．5℃，均温28．5℃）人工气候室中的“云烟87”为材料，运用液相色谱质谱联用法、分光光度法和荧光定量PCR法，研究了不同生长温度对烟叶生长发育过程中黄酮类化合物芸香苷和山奈酚-3-O-芸香苷含量、代谢相关酶活性及相关基因表达的影响。结果表明：随着烟草叶片的生长发育，黄酮类化合物代谢相关基因表达呈先降低后升高的趋势，从而调控了黄酮类化合物代谢相关酶活性的变化趋势为先降低后升高，使得黄酮类化合物中的芸香苷含量变化趋势为先降低后升高，山奈酚-3-O-芸香苷的含量则呈逐渐降低的趋势。此外，与生长在较高生长温度（均温23．5℃和均温28．5℃）下的叶片相比，较低生长温度（均温18．5℃）上调了烟草叶片类黄酮代谢相关基因PAL、C4H、4CL、CHS、F3H、F3’H和FLS的基因表达，提高了类黄酮代谢关键酶PAL、C4H、4CL和CHI的活性，促进了黄酮类化合物芸香苷和山奈酚-3-O-芸香苷的积累（p〈0．05），表明较低的生长温度（均温18．5℃）有利于黄酮类化合物在烟草叶片中的积累。

英文摘要：

In this study, the tobacco cultivar ＂Yunyan 87＂ was used as experimental material growed in artificial climate chamber with three different growth temperatures respectively （temperatures averaged 18.5 ℃, 23.5 ℃ and 28.5℃）. Through using liquid chromatography coupled with mass spectrometry, spectrophotometry and fluorescence quantitative PCR method to study the effects of different growth temperature on the contents of flavones rutin and kaempferol-3-O-rutin, the related enzyme activities and gene expression during the growth and development of tobacco leaves. The results showed that, with the growth and development of tobacco leaves, the expression of the genes related to flavonoids metabolism firstly decreased and then increased, and it regulated the related enzyme activities and the content of rutin firstly decreased and then increased. And make the content of flavonoids rutin firstly decreased and then increased, while kaempferol 3-O-rutin content was gradually decreased. In addition, compared with the tobacco leaves grew under higher temperature （temperatures averaged 23.5 ℃ and 28.5 ℃）, the tobacco leaves grew under lower temperature （temperature averaged l 8.5 ℃） enhanced the gene expression of PA L, C4H, 4CL, CHS, F3H, F3＇H and FLS, improved the key enzyme activities of PAL, C4H, 4CL and CHI, and finally promoted accumulation of the flavones rutin and kaempferol 3-O-rutin （p〈0.05）. It indicated that lower growth temperature （temperature averaged 18.5 ℃） is in favour of the accumulation of flavonoids in tobacco leaves.