中文摘要：

以小桐子(Jatropha curcas L.)cDNA为模版，克隆了JcGSK基因的CDS序列。序列分析表明,JcGSK基因包含1230bp完全阅读框(ORF)，编码409个氨基酸。预测其编码蛋白质的相对分子量为46.33kD，理论等电点为8.58。Blast搜索结果及进化分析结果表明，JcGSK蛋白与巴西橡胶树GSK蛋白的氨基酸序列一致性最高(94%)且亲缘关系最近；JcGSK基因编码的蛋白具有一个蛋白激酶特有的结构域。组织表达结果显示，JcGSK基因在小桐子根、茎、叶、花、果皮和种子中都有表达，且在根中表达量最高。小桐子幼苗在NaCl、ABA、PEG、低温和机械损伤处理后JcGSK基因表达量有不同程度的上调，推测其参与小桐子非生物胁迫响应和信号传导过程。JcGSK基因在种子中也有较高表达，在种子发育过程中表达量的变化与种子生长发育趋势基本一致，推测JcGSK基因也参与调控小桐子种子的生长发育。

英文摘要：

A complete CDS sequence of a glycogen synthase kinase family gene,JcGSK,was cloned from Jatropha curcas.JcGSK contained an opening reading frame(ORF)of1230bp,and encoded409amino acids.The predicted molecular mass was46.33kD with theoretical isoelectric point(PI)of8.58.Conserved domain analysis indicated that JcGSK had a typical domain of the GSK family.Multiple alignment and phylogenetic analysis showed that JcGSK had the highest identity(96%)and closest relationship with Hevea brasiliensis.Tissue expression results showed that JcGSK expressed significantly in different organisms and had the highest expression in root,NaCl,Abscisic acid(ABA),PEG,cold and mechanical damage upregulated JcGSK gene expression,indicated JcGSK involved in response abiotic stress and signal transduction process of J.curcas.JcGSK also highly expressed in seed.The expression level change of JcGSK in seed had the same trends with seed growth,indicated that JcGSK involved in regulation seed growth of J.curcas.