中文摘要：

目的 探讨siRNA抑制Mcl-1基因表达后对胃癌细胞株SGC7901生物学行为的影响.方法 化学合成针对Mcl-1的靶向siRNA（Mcl-1 siRNA组）,同时以转染阴性对照siRNA（阴性对照组）、转染脂质体转染试剂（脂质体对照组）和空白细胞（空白对照组）作为对照.MTT检测Mcl-1 siRNA对SGC7901细胞增殖能力的影响,AnnexinV-FITC和PI双染观察细胞各组凋亡情况;PI单染观察各组细胞周期变化情况;转染48 h后应用Transwell小室及Matrigel胶,通过计数细胞数目分析细胞侵袭迁移能力的变化.结果 MTT检测结果显示用Mcl-1 siRNA转染胃癌细胞SGC7901 24、48和72 h后Mcl-1 siRNA组与空白对照组、脂质体对照组、阴性对照组相比增殖能力显著下降（P〈0.05）;转染48 h后Mcl-1 siRNA组S期细胞明显增多,同时,G0/G1期、G2/M期比例相应减少,与各对照组比较差异有统计学意义（P〈0.05）;侵袭、迁移结果显示Mcl-1 siRNA组SGC7901细胞在转染后48 h穿膜细胞数均较各对照组明显下降（P〈0.05）.结论 Mcl-1在胃癌的发生、发展及侵袭转移中发挥重要作用,RNAi抑制Mcl-1是胃癌基因治疗的一个潜在有效的方法.

英文摘要：

Objective To investigate the effect of inhibiting Mcl - 1 gene expression on the biological behavior of gastric cancer cell line SGC7901 by using a small interference RNA （siRNA） strategy. Methods Synthesized siRNA targeting Mcl - 1 was transfected into SGC7901 cells, while SGC7901 cells transfected with negative siRNA and Lipofectamine 2000, and vacant SGC7901 cells were used as controls. MTF was adapted to investigate the proliferation of SGC7901 cells after transfection. After 48h transfeetion of Mel - 1 siRNA, the cells were stained by AnnexinV - FIFC and PI, as the flow eytometry was used to examine the apoptosis cells. Cell cycle was detected by FCM. Polycarbonate mem- brane transwell chamber and Matrigel were used for the detection of the alteration of invasion and migration capacity. Results The proliferation capacity of SGC7901 cells was significantly impaired after transfected with Mcl - 1 siRNA （P 〈 0. 05 ）. The S phase proportion in Mcl - 1 siRNA1 group was significantly higher than that in control groups （P 〈 0. 05 ）, with significant reduction in the G0/G1 and G2/M phases proportions 48 h after transfection. The results of Transwell chamber showed that the invasion and migration capacities of SGC7901 cells were also significantly impaired after transfected with Mcl - 1 siRNA （P 〈 0. 05）. Conclusion Mcl - 1 plays an important role in the pathogenesis of gastric cancer, providing a potential therapeutic target for gastric cancer.