目的研究假地蓝Crotalaria ferruginea全草的化学成分。方法采用硅胶柱色谱、Sephadex LH-20柱色谱进行成分分离纯化,UV、NMR、MS等方法进行结构鉴定。结果从假地蓝全草的醋酸乙酯萃取部分中分离并鉴定了12个化合物,分别为β-谷甾醇(Ⅰ)、高山金链花素(Ⅱ)、染料木素(Ⅲ)、正四十二烷酸(Ⅳ)、正三十二烷酸(Ⅴ)、正三十一烷醇(Ⅵ)、(2S,3S,4R,12E,2′R)-2-(2′-羟基-二十二碳酰胺基)二十碳-1,3,4-三羟基-12-烯(Ⅶ)、胡萝卜苷(Ⅶ)、白桦脂酸(Ⅸ)、3′,5-dihydroxy-4′methoxy-2″,2″-dimethylpyrano-(5″,6″,6,7)-isoflavone(Ⅹ)、12-oleanene-38,22β,24-triol(soyasapogenol B,Ⅺ)、laburnetin[4′,5,7-trihydroxy-6-(2-hydroxy-3-methyl-3-butenyl)isoflavone,Ⅻ]。结论所有化合物均为首次从该植物中分离得到。
Objective To study the chemical constituents of Crotalaria ferruginea. Methods The compounds were isolated by silica gel and Sephadex LH-20 chromatography and their structures were iden- tified by the spectra evidences of UV, NMR, and MS. Results Twelve compounds were isolated from ethyl acetate extract and their structures were identified as β-sitosterol (Ⅰ ), alpinumisoflavone (Ⅱ ), genistein (Ⅲ ), dotetracontanoic acid ( Ⅳ), dotriacontanoic acid ( Ⅴ ), 1-hentriacontanol (Ⅵ ), (2S, 3S, 4R, 12E, 2′R)-2-(2′-hydroxyl-docosanoylamino)eicosane-1, 3, 4-trihydroxy-12-ene (Ⅶ), daucosterol (Ⅷ), betulinic acid (Ⅸ), 3′, 5-dihydroxy-4′-methoxy-2″, 2″-dimethylpyrano-(5″, 6″, 6, 7)-isoflavone (Ⅹ), 12-oleanene-3β, 22β, 24-triol (soyasapogenol B, Ⅺ )and laburnetin [4′, 5, 7-trihydroxy-6-(2- hydroxy-3-methyl-3-butenyl) isoflavone, Ⅻ]. Conclusion All these compounds are reported in this plant for the first time.