中文摘要：

目的研究超低频经颅磁刺激（TMS）对脑缺血大鼠认知功能的影响并探讨其机制。方法将60只健康大鼠分为4组（各15只）：A组（假手术组）,B组（模型组）,C组（TMS组）和D组（TMS＋H89组）。比较各组大鼠的逃避潜伏时间、跨越原平台次数及血管内皮生长因子（VEGF）、脑源性神经营养因子（BDNF）和巢蛋白（nestin）的表达水平。结果 A组大鼠逃避潜伏期为（16.31±2.33）s,跨越原平台次数为（8.02±1.76）次;B组大鼠逃避潜伏期为（57.14±2.89）s,跨越原平台次数为（3.15±0.88）次;C组大鼠逃避潜伏期为（29.18±1.95）s,跨越原平台次数为（5.44±0.75）次;D组大鼠逃避潜伏期为（45.87±2.06）s,跨越原平台次数为（4.16±1.02）次。与A组比较,B、C、D组大鼠的逃避潜伏期明显延长,且B组长于D组及C组,差异均有统计学意义（P〈0.05）;大鼠的跨越原平台次数明显减少,且B组少于D组及C组,差异均有统计学意义（P〈0.05）。各组大鼠VECF、BDNF和nestin表达水平比较,差异均有统计学意义（P〈0.05）。结论低频TMS可以明显改善脑缺血大鼠的认知功能,其作用与环磷腺苷效应元件结合蛋白及其下游基因（VEGF、BDNF）的表达有关。

英文摘要：

Objective To study the influence of ultralow-frequency transcranial magnetic stimulus（TMS）on the cognitive ability of rats with cerebral ischemia and its mechanism.Methods 60 healthy rats were divided into 4groups（15cases each）：A（sham-operation）,B（model）,C（TMS）and D（TMS＋H89）.The escape latency time,times of passing through platform,expression level of VEGF,BDNF and nestin protein were compared among 4groups.Results In the group A,the escape latency time was（16.31±2.33）s,times passing through platform were（8.02±1.76）times;in group B,which were（57.14±2.89）s and（3.15±0.88）times;in group C,which were（29.18±1.95）s and（5.44±0.75）times;in group D,which were（45.87±2.06）s and（4.16±1.02）times.Compared with the group A,the escape latency time in the group B,C and D was significantly extended,moreover that in the group B was longer than that in the group D and C,the differences were statistically significant（P〈0.05）;the times of passing through platform decreased,which in the group B was less than that in the group D and C,the differences had statistical significance（P〈0.05）.The expression levels of VEGF,BDNF and nestin had statistical differences among various groups（P〈0.05）.Conclusion Low-frequency TMS can significantly improve the cognitive ability of rats with cerebral ischemia,its effect is related to the expression of cAMP-response element binding protein and its following genes（VEGF and BDNF）.