中文摘要：

目的探讨丹参多酚减轻心肌细胞损伤的作用及机制。方法将原代培养的成年小鼠心肌细胞分为4组：对照组（换MEM细胞培养液）、丹参多酚组（用含有5g/L丹参多酚的MEM细胞培养液处理细胞3小时）、H2O2组（用含有25μM H2O2的MEM细胞培养液处理细胞3小时来诱导成年小鼠心肌细胞的损伤）及丹参多酚＋H2O2组（用同时含有5g/L丹参多酚及25μM H2O2的MEM细胞培养液处理细胞3小时）。检测4个不同处理组中细胞活力、细胞内ATP水平、线粒体体膜电位的变化。结果与对照组相比,H2O2组中的细胞存活率明显下降,细胞内ATP水平显著降低,线粒体膜电位也明显下降（均P〈0.05）。而丹参多酚能明显抑制H2O2引起的细胞存活率下降、细胞内ATP水平的降低以及线粒体膜电位的下降（均P〈0.05）。结论丹参多酚可以对心肌细胞起到保护作用,其发挥保护作用的机制可能与其减轻线粒体功能障碍有关。

英文摘要：

Objective To investigate the protective role and mechanism of salvianolate on cardiac myocytes injury.Methods The primary cultured adult mouse cardiomyocytes were divided into control group,salvianolate group,cardiomyocytes exposed to MEM containing 5g/L salvianolate for 3hours.H2O2 group,cardiomyocytes exposed to MEM with25μM H2O2 for 3hours and the salvianolate ＋ H2O2 group,cardiomyocytes were treated with MEM containing 5g/L salvianolate and 25μM H2O2.Then,the survival of cardiomyocytes was counted.The intracellular ATP and the mitochondrial membrane potential were determined in these groups.Results Compared with control,the survival of cardiomyocytes was significantly decreased and the intracellular ATP and mitochondrial membrane potential were also significantly decreased in the H2O2group（P〈0.05）.However,these effects of H2O2 on cardiomyocytes were abolished by salvianolate（P〈0.05）.Conclusion Salvianolate could alleviate adult mice cardiomyocytes injury by preventing mitochondrial dysfunction.