中文摘要：

氧化亚氮（N2O）是BioDeNOx脱硝过程中的主要中间产物,而N2O还原酶是影响NO进行完全反硝化的关键酶。实验以前期从同一反应体系中筛选出的一株反硝化菌Pseudomonas stutzeri ND1为出发菌株,克隆鉴定该反硝化菌是否具有N2O还原酶基因nosZ,并考察了pH、氧气、温度对该反应体系NO去除率以及N2O产生量、转化率的影响。实验证明该菌确实存在N2O还原酶nos,即从微生物学角度表明该反应体系具有N2O的生物还原能力。该反应体系的最适pH值为8.0,此时NO去除率达最大值80%,N2O产量降至0.33mg/m^3,N2O转化率为0.06%;在氧气含量小于1%时,N2O产量及转化率变化不明显,NO去除率下降了不到2%,而当氧气含量达到2%时,N2O有了明显的跃升,达到1.5 mg/m3,N2O转化率为0.48%,NO去除率下降至78.6%;反应最适温度为30℃,此时NO去除率达到最大值82%,N2O产量为0.34 mg/m^3,且N2O产量随着温度的增加而逐渐增多。

英文摘要：

Nitrous oxide（N2O） is the main intermediate product in the BioDeNO, and N2O reductase is the key enzyme in the biological denitrifying process. By using a selected denitrifying bacteria of Pseudomonas stutzeri ND1, whether there was N2O reductase gene was identified, and effects of process parameters such as pH, O2, temperature on N2O production, as well as NO removal efficiency in the biological denitrifying process were studied. Results showed that the system really has nosZ, which indicated that the reaction system has a capacity of biological reduction of N2O with a microbiological point of view. The optimum pH of the reaction system was 8.0, then the maximum NO removal rate of 80%, N2O production fell to 0.33 mg/m3, N2O conversion rate of 0.06%. When the oxygen content was less than 1%, N2O production and the conversion rate didn＇t change significantly, with NO removal efficiency decreased less than 2%, and when the oxygen content reach 2%, it was found N2O production had a marked jump and reached 1.5 mg/m^3, the rate of N2O conversion was 0.48%, and NO removal rate decreased to 78.6%. Under the optimum reaction temperature 30℃, NO removal rate reached a maximum 82%, N2O production was 0.34 mg/m^3, and N2O production increased as the temperature gradually increase.