采用序批式污泥培养方式,探讨了NO2ˉ为电子受体的反硝化除磷工艺特征.结果表明,通过逐步增加进水中NO2ˉ浓度并取代NO3ˉ,可有效驯化反硝化聚磷菌(DPAO)对较高浓度NO2ˉ(以N计,30mg/L)的有效利用.DPAO可根据电子受体的变化作出动态响应,进而影响系统的除磷效能.在NO3ˉ条件下,系统中存在2类DPAO:DPAO35(以NO2ˉ或NO3为电子受体)和DPAO5(以NO3ˉ为电子受体);作为系统的优势功能菌,DPAO占总VSS的40%-61%.随着进水中NO3ˉ的减少和NO2ˉ的增加,DPA05被缓慢淘洗出系统,DPAO比例减少为28%'-4-1%,同时引起聚糖菌(GAO)比例从31%~52%升至54%~67%,而除磷效率从84%降至66%.化学计量学显示厌氧△Gly/△HAc和好氧AGly/△PHA比值(C/C)分别由0.53和0.43升至0.78和0.51.
Process characteristics of denitrifying dephosphatation with NO2ˉ as electron acceptor was investigated by adopting batch sludge culture. Through stepwise elevation of NO2ˉ instead of NO3ˉN added in influent, denitrifying poly-P accumulating organism (DPAO) was gradually accommondated to utilize NO2 with high concentration of 30mg/L. During this period, DPAO had a dynamic response according to the change of electron acceptor, and it ultimately determined the performance of P removal. Under the conditions of NO3ˉ, two groups of DPAO existed and dominated in the system (40%-61% of VSS), namely DPAO35 and DPAOs, and they were able to denitrify NO2 and NO3ˉ or NO3ˉ only as respective electron acceptor. With the decrease of NO3ˉ and simultaneous increase of NO2ˉ, DPAO5 was excluded from the system and DPAO ratio therefore dropped down to 28%-41%.However, glycogen accumulating organism (GAO) grew to dominance in the reactor from 31%-52% to 54%-67%, and it thus led to in decrease of P removal from 84% to 66%. Accordingly, stoichiometry coefficients of △Gly/△HAc (anaerobic phase) and AGly/△PHA (aerobic phase) increased from 0.53 and 0.43 to 0.78 and 0.51 (C/C), respectively.