中文摘要：

以文冠果壳为试材,采用超声振荡的方法,研究了超声温度、超声功率、超声时间、料液比对文冠果壳粗提物提取率的影响,通过正交实验确定最佳提取工艺条件。依次通过硅胶H柱、ODS-A反相柱对总皂苷进行分离纯化,流动相分别为甲醇乙酸乙酯、甲醇水溶液,并选用3种细菌和2种真菌考察抑菌活性。结果表明：最佳提取工艺条件是超声温度70℃,超声功率180W,超声时间30min,经HPLC分析,料液比1∶25g·mL^（-1）,提取率为17.26%,对提取率的影响顺序为料液比〉超声时间〉超声功率〉超声温度。80%甲醇馏分的文冠果壳苷含量和纯度最高,该馏分对金黄色葡萄球菌和白色念珠菌有抑制作用。该研究为文冠果壳苷的提取纯化工艺优化,抑菌活性的开发提供了理论基础。

英文摘要：

Taking Xanthoceras sorbifolia Bunge. shell as material, effects of materials liquid ratio （ML）, ultrasound temperature （UTe）, ultrasound time （UTi） and ultrasound intensity （UI） on extraction rate of crude extract were studied and the optimize extraction conditions were done by orthogonal experiment. Then the xanthoceraside was separated and purified through silica gel H and ODS-A column. Finally, to research the anti-microbial activities of different fractions from purifications, three kinds of bacteria and two kinds of fungi were collected. The results showed that the optimize extraction conditions were that the UTe, UI, UTi, SR was 70 ℃, 180 W, 30 minutes, 1 ： 25 g·mL-1, respectively;the optimize extraction rate was 17. 26%. The content and purity of xanthoceraside was the highest in the 80% methanol-water fraction, which anti-microbial activities were presented on Staphylococcus aureus and Candida albicans. Some theoretical basis were provided for the optimization of the extraction and purification techniques and the research of anti-microbial activities of xanthoceraside.