中文摘要：

【目的】探讨白藜芦醇衍生物白藜芦醇亚甲胺﹙resveratrol methyleneimine,RMI﹚对小鼠酒精性脂肪肝的作用及其作用机制。【方法】通过油红O染色观察RMI对氧化低密度脂蛋白（ox-LDL）诱导的RAW264.7巨噬细胞脂质蓄积的影响;建立小鼠酒精性脂肪肝模型,分别以RMI﹙5 mg/kg和10 mg/kg﹚灌喂6周后,检测酒精性脂肪肝模型小鼠肝脂质、血脂,血清中丙二醛（MDA）、总抗氧化能力（T-AOC）、谷丙转氨酶（ALT）、谷草转氨酶（AST）的含量,以及肝组织中MDA、超氧化物歧化酶﹙SOD﹚和谷胱甘肽过氧化物酶﹙GSH-Px﹚的含量,同时观察肝组织切片病理变化。【结果】RMI可抑制ox-LDL诱导的RAW264.7巨噬细胞脂质蓄积。RMI组小鼠血清中总胆固醇﹙TC﹚、甘油三酯﹙TG﹚、低密度脂蛋白胆固醇﹙LDL-C﹚和肝组织中TC、TG和MDA的含量较模型组显著降低（均P〈0.05）;而血清中的高密度脂蛋白胆固醇﹙HDL-C﹚、T-AOC,肝组织中SOD、GSH-Px含量较模型组显著升高（P〈0.05或P〈0.01）;肝组织切片观察显示RMI组与模型组比较,肝细胞脂肪变性程度减轻,脂肪粒减少。【结论】RMI可能通过降低脂质过氧化物、增加机体抗氧化能力,而对酒精性脂肪肝有一定的改善作用。

英文摘要：

Objective To study the effects of resveratrol methyleneimine（RMI）, a resveratrol imine derivative（RES）, on mice alcoholic fatty liver（AFL） and to explore its possible mechanism. Methods Oil red O staining was used for observing the effect of RMI on RAW264.7 macrophage lipid accumulation induced by oxidized lowdensity lipeprotein（ox-LDL）. Mice with alcoholic fatty liver were fed orally with RMI at the dose of 5 and 10 mg/kg for 6 weeks, and then the lipid levels in hepatic tissue and blood, and the serum contents of malondialdehyde（ MDA）, total antioxidant capacity（ T-AOC）, alanine aminotransferase（ ALT）, and aspartate aminotrasferase（AST） as well as the contents of MDA, superoxide dismutase（SOD） and glutathione peroxidase（GSH-PX） in the liver tissue were measured. Meanwhile, the histological changes of hepatic tissue were observed. Results RMI inhibited RAW264.7 macrophage lipid accumulation induced by ox-LDL. After fed with RMI, the model mice had lower serum levels of total cholesterol（TC）, triglyceride（TG） and low density lipoprotein-cholesterol（LDL-C）, and also had lower hepatic TC, TG and MDA levels than the model group（P〈0.05）. Serum high density lipoprotein-cholesterol（ HDL-C） and T-AOC level, and hepatic SOD a nd GSH-PX were increased in RMI groups as compared with the model group（ P〈0.05 or P〈0.01）. Moreover, the results of hepatic histological examination showed that the fatty degeneration in liver cells was alleviated, and the fat particles were reduced in RMI groups as compared with the model group. Conclusion RMI could reduce lipid peroxides and increase the anti-oxidation activtiy, thereby having certain effect on improving alcoholic fatty liver.