中文摘要：

应用SSRHunter软件对NCBI公共数据库中的猕猴桃EST序列进行筛查，利用Primer5．0软件设计引物并进行筛选，同时对其在部分柑橘类植物的通用性进行分析。以漓江猕猴桃DNA为模板，对所设计的97对EST-SSR引物进行筛选，结果表明其中77对引物能扩增出清晰条带，有效扩增率为79-38％。随机选取20对引物对10份猕猴桃资源进行检测，结果发现有17对引物对所有材料都有扩增产物并呈现出多态性，多态性扩增率为85％。随机应用20对有效EST-SSR引物对兴津、金柑、枳壳、桠柑、酸柚、甜橙、胡柚、尾张、宫川等柑橘类植物基因组DNA进行扩增，结果表明，其中有11对引物在供试材料中有扩增产物，占引物总数的55％：有8对引物的扩增产物具有多态性，扩增率为72．7％。据此，基于猕猴桃EST序列而筛选的SSR引物在柑橘类植物中具有一定的通用性。

英文摘要：

ESTs of Actinidia in the NCBI database were downloaded and screened by SSRHunter software, the EST-SSR primers were designed by Primer 5.0, and their transferabilities were analyzed in some Citrus plants. The 97 EST-SSR primers which were deigned were mined by using genomic DNA of Actinidia lijiangensis. The results indicated that the 77 pairs of primer showed amplification, accounting for 79.38%. Twenty pairs of primer selected were detected to PCR for DNAs from 10 A ctinidia varieties, the 17 primer pairs of the 20 showed amplification and polymorphism, accounting for 85%. The transferability of 20 pairs of EST-SSR primers which were randomly selected was explored in 9 Citrus germplasms （Okit-su, Kumquat, Trifoliate Orange, Ponkan, Sour Pummelo, Sweet Orange, Huyou, Owari, Miyagawa）. The results showed that the 11 primer pairs of the 20 tested primers had the amplification, accounting for 55%. And the 8 primer pairs showed polymorphism, accounting for 72.7%. The results revealed that the EST-SSR markers in A ctinidia were transferable in some Citrus germplasms.