中文摘要：

黑色素皮质激素受体1 （melanocortin-1 receptor, MC1R）基因在哺乳动物毛色调控过程中起重要作用，但外源MC1R基因对羊驼（Lama pacos）毛囊干细胞（hair follicle stem cells, HFSCs）中小眼畸形相关转录因子（micropht-halmiaassocitated transcription factor, MITF）和酪氨酸酶（tyrosinase, TYR）基因表达特性的影响研究较少。为了探讨MC1R基因对MITF和TYR mRNA表达的影响，本研究通过构建羊驼MC1R真核表达载体pcDNA3.1（＋）-MC1R，瞬时转染HFSCs，qRT-PCR检测转染前后MC1R、MITF、TYR mRNA表达量；并采用细胞免疫化学技术对MC1R进行细胞定位。qRT-PCR结果显示，羊驼HFSCs转染pcDNA3.1（＋）-MC1R后，MC1R mRNA表达量显著增加（P＜0.05），分别是空载体组和对照组的2.15和2.26倍，MITF和TYR mRNA表达量增加不显著（P＞0.05）；细胞免疫化学结果显示，MC1R在羊驼HFSCs胞浆中呈阳性表达。结果表明，MC1R在HFSCs中高效表达，间接上调MITF和TYR基因的表达。研究结果为进一步研究MC1R对羊驼HFSCs表达毛色蛋白的作用机理提供科学基础。

英文摘要：

Melanocortin-1 receptor （MC1R） gene may play important roles in the regulation process of mammalian coat color, but the effects of exogenous MC1R gene on the micropht-halmiaassocitated transcription factor （MITF） and tyrosinase （TYR） genes in alpaca （Lama pacos） are less studied. To explore effect of MC1R gene on the expression of MITF and TYR, eukaryotic expression vector of MC1R gene pcDNA3.1（＋）-MC1R was constructed and transfected into alpaca hair follicle stem cells （HFSCs）. The relative expression of MC1R, MITF and TYR mRNA were detected by qRT-PCR, and MC1R protein was located by immunocytochemistry. qRT-PCR results showed that the relative expression level of MC1R was significantly increased （P〈0.05） in the group transfected with pcDNA3.1（＋）-MC1R （P〈0.05）. There were 2.15 and 2.26 times than that in the group of pcDNA3.1（＋） and the control group. MITF and TYR gene also had higher expression, but not significant （P〉0.05）. Meanwhile immunocytochemistry results showed that MC1R protein had a positive expression in HFSCs. The results showed that the MC1R gene excessively expressed in alpaca HFSCs, and indirectly up-regulated the expression of TYR and MITF. This study provides scientific basis for further study on the expression mechanism of MC1R in the alpaca HFSCs.