中文摘要：

microRNAs (miRNAs ) 表示侧面在 DNA 损坏下面在房间显示出实质的变化，这被知道。这里，我们做了 miRNA microarray 和量的即时 PCR 包括地鉴别差别在结肠癌房间线 HCT116 p53 表示了 miRNAs < 啜 class= “ a-plus-plus ” >+/+ 和 HCT116 p53 < 啜 class= “ a-plus-plus ” >/ 。簇分析表明差别的一块面板表示了被 p53 调整的 miRNAs 或 UV-C 导致了 DNA 损坏。这些改变的 miRNAs 趋于位于染色体 13， X 并且 17。而且，小径丰富分析估计那条 MAPK 小径，焦点的 adheren 小径， p53 小径和 Wnt 小径被这些 miRNAs 调停在 DNA 损坏反应施加他们的功能。另外，我们发现了那 miR-320a， UV-C 之一导致了 miRNAs，在保护房间免受 DNA 的伤害起一个作用损坏。一起拿，我们 miRNAs 是动态的结果表演在不同房间上下文和不同状况列在后面 DNA 损坏在 p53 依赖或独立的礼貌调整了。

英文摘要：

It is known that microRNAs （miRNAs） expression profile shows substantial changes in cells under DNA damage. Here, we did miRNA microarray and quantitative real-time PCR to comprehensively identify the differentially expressed miRNAs in colon cancer cell lines HCT116 p53＋/＋ and HCT116 p53-/-. Cluster analysis revealed a panel of differentially expressed miRNAs which are regulated by p53 and/or UV-C induced DNA damage. These altered miRNAs tend to be located in chromosomes 13, X and 17. Moreover, pathways enrichment analysis estimated that MAPK pathway, focal adheren pathway, p53 pathway and Wnt pathway were mediated by these miRNAs to exert their functions in DNA damage response. Additionally, we found that miR- 320a, one of the UV-C induced miRNAs, play a role in protecting cells from DNA damage. Taken together, our results show that miRNAs are dynamic regulated in p53- dependent or -independent manners in different cell contexts and different situations following DNA damage.