中文摘要：

为研究驻极体外静电场对猪胰岛素结构和介电特性的影响，从而为进一步研究驻极体提高胰岛素的降糖能力提供理论依据，以常温电晕充电下制备得到的500V、1000V和1500V聚丙烯驻极体为电场源作用于胰岛素，测量药物的d33系数及核磁共振和非变性凝胶电泳谱。研究结果表明：1）不同表面电位聚丙烯驻极体产生的外静电场能使胰岛素溶液和胰岛素贴剂两侧形成相对稳定的静电场，电场强度与驻极体的等效表面电位成正比；2）驻极体外静电场对胰岛素的一级结构和二级结构没有明显影响；3）经500V、1000V和1500V驻极体作用的胰岛素贴剂的d33值分别较胰岛素贴剂提高15．7倍、21．3倍和32．0倍；4）核磁共振谱表明驻极体作用后的胰岛素结构中氢键网络发生了改变。因此，驻极体产生的静电场在一定程度上引起了胰岛素分子的极化，改变了胰岛素的带电性，有利于胰岛素单体的形成，并能够提高胰岛素的降糖能力。

英文摘要：

In order to study the effects of external electrostatic field of electret on structure and dielectric property of procine insulin, and to provide theory evidence for the study of the electret to increase the effect of insulin on blood glu- cose reducing, the corona charged 500 V, 1 000 V, and 1 500 V polypropylene （PP） electrets were acted on insulin as electric field source. The IH-NMR spectroscopy, gel electrophoresis, and piezoelectric d33 coefficient were determined, respectively. The results show that： 1） PP electret with different surface potential can produce stable electrostatic field on both sides of the insulin solution or insulin patch. The higher the surface potential of the electret is, the stronger the elec- trostatic field would be. 2） The external electrostatic field of PP electret has no obvious effects on the primary and secondary structures of insulin. 3） The piezoelectric d33 coefficients for insulin patches treated by 500 V, 1 000 V, and 1 500 V PP electrets are 15.7, 21.3, and 32.0 folds more than those of the non-electret treated insulin patch. 4） The -H-NMR spectroscopy indicates that the hydrogen network of insulin structure is changed after the action of electret. So, electrostatic field produced by PP electret leads to the weak polarization of the insulin molecules, alters the charging characteristic of the insulin, and it is helpful for the formation of insulin monomers. Therefore, electrets can increase the effect of insulin on blood glucose reducing.