中文摘要：

利用SMART技术构建了北京油鸡肝脏全长cDNA文库,构建的未扩增文库滴度为1.96×106pfu·mL-1,文库重组率为92.8%,插入片段长度为1.23kb,扩增文库滴度为1.64×1010pfu·mL-1。设计purH基因保守引物对该文库进行筛选,克隆了北京油鸡purH基因（GenBank登录号：EU334506）,将purH基因完整开放阅读框定向插入pEGFP-N3,构建带有GFP报告基因重组表达载体pEGFP-N3-purH,将其导入北京油鸡体外培养细胞中。转染后24、48和72h,pEGFP-N3-purH转染率为10.3%～53.2%,绿色荧光均匀分布于细胞质与细胞核中。随表达量的增加,绿色荧光在细胞核中聚集成团块状或颗粒状。结果表明,构建的北京油鸡肝脏组织cDNA文库符合建库要求。

英文摘要：

A cDNA library from Beijing fatty chicken liver tissue was constructed according to the protocol of SMARTTM cDNA library construction kit.The titer of the initial library was 1.96 × 106 and 1.64 × 1010 pfu·mL-1 respectively.The percentage of recombinants was 92.8% and average length of exogenous inserts was 1.23 kb.The full-length sequence of a purH gene （GenBank accession No：EU334506） was successfully amplified from the library using a pair of conserved primers.Full-length purH cDNA was inserted into the pEGFP-N3 vector and transfected into Beijing fatty chicken fibroblasts.The expression efficiency ranged from 10.3% to 53.2% in 24,48 and 72 h after transformation,and green fluorescence was distributed throughout the cytoplasm and nucleus except in the cryptomere vesicle.A high quality cDNA library from Beijing fatty chicken was successfully constructed and provided a useful resource for the functional genomic research of Beijing fatty chicken.