目的:比较不同方法对宫颈拭子样本沙眼衣原体的检测效果。方法:采集300例20~50岁无感染症状女性宫颈分泌物,分别采用细胞培养、聚合酶链式反应及胶体金法进行沙眼衣原体检测,培养阳性或两个非细胞培养方法检测为阳性,定为真阳性,称为扩大金标准。结果:300例受检查者,按扩大金标准CT感染者共36例,发病率为12%(36/300),细胞培养、聚合酶链式反应、胶体金法敏感性分别为72.22%、91.67%、52.78%。特异性分别为100%、98.11%、98.86%。阳性预测值分别为100%、86.84%、86.36%。阴性预测值分别为96.35%、98.85%、93.88%。结论:三种检测方法均可用于CT感染的诊断。
Objective:To investigate the effect of different testing methods in diagnosis of Chlamydia Trachomatis (CT) from cervical swabs. Method:Cervical swabs were collected from 300 asymptomtic cases and the CT was detected by cell culture, polymerase chain reaction(PCR) and Colloidal Gold. The sample from which cell culture is positive or other two methods are positive, defined as true positive or expanding gold standard. Results: According to the expanding gold standard , there were 36 cases diagnosed with CT infection. The chlamydia positivity was 12 %(36/300). For cell culture, PCR and Colloidal Gold methods, the sensitivity was 72. 22 %, 91. 67 %and 52. 78 % , respectively, and the specificity were 100 %,98. 11 %and 98. 86 % respectively. The positive predicting value of cell culture, PCR, and Colloidal Gold was 100 %,86. 84 % and 86. 36 %, respectively, while the negative predicting value was 96. 35 %, 98. 85 %and 93. 88 % respectively. Conclusion: The three testing methods can all be used to diagnosis the CT infection.