中文摘要：

从天山雪莲叶片低温诱导的eDNA文库中克隆了一个未知蛋白基因（SikSD-82）eDNA全长序列。生物信息学分析表明SikSD-82基因共编码262个氨基酸，具有小麦铝诱导蛋白Wali7的保守结构域，属Gn—AT-Ⅱ家族的可溶性蛋白；其氨基酸序列与蓖麻的茎部特异性表达蛋白相似性最高为60．89％；进化树分析显示，该蛋白与番茄的茎部特异性表达蛋白关系最近。亚细胞定位结果表明，SikSD-82蛋白主要定位于细胞核。在铝胁迫条件下，转SikSD-82烟草幼苗根的伸长明显优于野生型，根部铝离子和丙二醛含量均显著低于野生型烟草，转SikSD-82烟草显示出较强的耐铝性。

英文摘要：

A unknown function protein gene SikSD-82 was cloned from Saussurea involucrata Kar. et Kir. eDNA library under cold-stress. Physieoehemical properties and molecular features of SikSD-82 were pre- dicted by bioinformatic analysis showed that SikSD-82 gene encoding a stable protein with 262 amino acids,which contains a conserved WaliT（wheat aluminum induced protein） ,a member of Gn_AT_ 1I super- family, had significant identity of 60.89 ~ with stem-specific proteins from Ricinus communis. Phylogenetic analysis indicated that SikSD-82 protein was most closely related to Solanum lycopersicum stem specific protein. The subcellular localization of SikSD-82 was found mostly in the nucleus . Under aluminum stress,SikSD-82 transgenic plant accumulate less A1 and MDA concentration in roots than that of wild- type plants,and their roots are longer than that of wild-type plants~ ,which shows that SikSD-82 transgenic plants have more aluminum tolerance than that of wild-type plants.