中文摘要：

以天山雪莲（S.involucrata Kar.et Kir.）为材料,根据已获得的天山雪莲1,5-二磷酸核酮糖羧化/加氧酶小亚基基因组序列设计引物,采用热不对称交错PCR（TAIL-PCR）和高效TAIL-PCR（hiTAIL-PCR）扩增到rbcS基因5′上游启动子序列,长为1 668bp。用PlantCare和PLACE软件序列分析表明,该序列具有启动子的基本元件TATA-box、CA AT-box,包含多个胁迫诱导元件,如光诱导元件、赤霉素、低温诱导元件,昼夜节律调控元件等。构建pBI-PsikrbcS-GUS植物表达载体,并成功将其转化进入农杆菌。sikrbcS基因启动子的克隆与分析为进一步研究雪莲1,5-二磷酸核酮糖羧化/加氧酶小亚基基因的表达调控奠定了基础。

英文摘要：

Based on the known sequence of rbcSgene we designed nested specific primers,and the promoter sequence of ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit gene fromSaussurea involucrata Kar.et Kir.genomic DNA was obtained by TAIL-PCR and hiTAIL-PCR.Nucleotide sequencing analysis showed that the full length of the fragment is 1 668bp.The functional elements were analyzed by PLACE and Plant Care programs.The promoter sequence contains the basic elements：TATA-box,CAAT-box and stress-induced elements,light responsive element,gibberellin responsive element,low-temperature responsiveness element and regulatory element involved in circadian control.The plant expression vector pBI-PsikrbcS-GUS was constructed,and then transformed into agrobacteria successfully.Cloning and analyzing of the promoter region of sikrbcSgene made substantial basis for further research on the mechanism of regulation and expression of Saussurea involucrata Kar.et Kir ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit gene.