中文摘要：

利用电喷雾质谱方法（ESI-MS）分析了附子加辅料（甘草）炮制前后水煎液中二萜类生物碱在种类和含量方面的变化，通过加入内标化合物，建立了电喷雾质谱的半定量分析方法。此方法具有快速、准确、灵敏的特点，能够更加全面地反映中药配伍炮制过程中多种化学成分的含量变化，并能根据电喷雾串联质谱的分析结果鉴定配伍后产生的新的化学成分，在共煎液中的次乌头碱、中乌头碱和乌头碱的相对含量分别是单煎液中的5．67％、4．05％和4．88％。通过研究附子与甘草的单煎液、共煎液以及药渣中化学成分的变化，揭示了甘草作为辅料，在炮制过程中对附子减毒作用机理。

英文摘要：

Norditerpenoid alkaloids in the processing of Radix aconiti lateralis preparata with Radix glycyrrhizae preparata was studied by electrospray ionization tandem mass spectrometry. A method of ESI-MS couple with internal standard was applied for semiquantitative analysis of norditerpenoid alkaloids before and after processing. The combined-decoction-to single-decoction-ratios of the relative abundance of toxic hypaconitine, mesaconitine and aconitine were 5. 67%, 4. 05% and 4. 88%, respectively. The chemical changes of processing can been comprehensive observed by ESI-MS/MS analysis. The scientific basis of reducing the toxicity of Radix aconiti lateralis preparata with Radix glycyrrhizae preparata was enlightened by compareing with the single-decotion, the combined-decoction and residue for the compatibility of Radix aconiti lateralis preparata and Radix glycyrrhizae preparata.