中文摘要：

目的探讨高浓度葡萄糖条件下胰岛细胞胰岛素受体底物2(IRS2)和Bax基因表达和激活情况以及对诱导胰岛细胞凋亡的影响。方法将小鼠胰腺进行分离纯化所得的胰岛细胞进行体外培养,按培养液里葡萄糖浓度不同分为G1组(5.6mmol/L)、G2组(7.8mmol/L)、G3组(11.1mmol/L)、G4组(16.7mmol/L)、G5组(22.2mmol/L)及G6组(27.6mmol/L)。细胞培养至72h后,采用放射免疫法检测各组胰岛细胞胰岛素分泌水平、免疫细胞组织化学法检测IRS2表达、免疫荧光法检测细胞Bax激活和细胞核Hoechst33342染色检测细胞凋亡情况。结果当葡萄糖浓度在5.6～11.1mmol/L时,胰岛素分泌水平升高,IRS2表达和Bax激活分别呈进行性增加,但此时细胞凋亡率无明显改变。随着葡萄糖浓度升高至超过16.7mmol/L时,IRS2表达逐渐减少,胰岛素分泌量明显降低,而Bax激活则逐渐增强,胰岛细胞凋亡率呈明显增加趋势。结论高浓度葡萄糖可以导致胰岛细胞IRS2表达下降,胰岛素分泌量下降;同时Bax激活增强,胰岛细胞凋亡率也明显增加。说明IRS2表达下降与凋亡相关蛋白Bax激活及其胰岛...

英文摘要：

Objective To investigate the role of insulin receptor substrate 2(IRS2) and Bax on mouse islet cell apoptosis in the presence of high glucose in vitro.Methods The pancreatic islet cells were isolated from Kunming mice and divided into 6 groups(G1-G6 groups) for a 72-h culture in the media containing different concentrations of glucose(5.6,7.8,11.1,16.7,22.2,and 27.6 mmol/L,respectively).Insulin secretion by the cells was evaluated by radioimmunoassay,and the expressions of IRS2 and Bax were detected using i...