中文摘要：

目的研究罗格列酮在不同浓度葡萄糖条件下对B细胞株NIT—1细胞增殖、凋亡及胰岛素分泌的影响。方法将NIT-1细胞按5×10^4个细肜孔至24孔培养板中培养，根据培养基RPM1640葡萄糖浓度不同分为以下5组：G1组（5．6mmol／L）、G2组（11．1mmol／L）、G3组（16．7mmol／L）、G4组（22．2mmol／L）及G5组（27．6mmol／L），继续培养72h后，分别给予10^-5mmol／L罗格列酮干预48h后，取上清液采用放免法检测各组胰岛素水平；采用免疫荧光法检测凋亡细胞，MTT法检测细胞增殖情况。结果（1）罗格列酮能够明显抑制高浓度葡萄糖诱导的NIT-1细胞凋亡。施加罗格列酮浓度10^-5mmol／L48h时后，能够明显抑制16．7mmol／L、22．5mmol／L和27．6mmol／L浓度的葡萄糖所诱导的细胞凋亡。（2）罗格列酮能显著增加各浓度葡萄糖组NIT-1细胞的增殖活性。（3）罗格列酮能显著增加各葡萄糖浓度组的NIT—1细胞胰岛素分泌。结论罗格列酮可以通过直接作用于胰岛B细胞调节改善的胰岛B细胞的增殖及功能、抑制凋亡。

英文摘要：

Objective To explore the roles of rosiglitazone in islet B cells lines （NIT-1 cells） proliferation,apoptosis and secretion induced by high concentration glucose in vitro. Methods The NIT-1 cells were cultured with different concentrations of glucose in RPM1640 in 24 well plates at a density of 5 × 104, and divided into the following groups：G1, G2, G3, G4 and GS, with glucose of 5.6,11.1,16.7,22.5 and 27.6 mmol/L,respectively. After culture for 72 hours, all the cells were treated with 10^-5 mmol/L rosiglitazone for 48 hours, then cells insulin level was evaluated by radioimmunoassay ,cells apoptosis was examined by Hoechst 33342 assay ,and cells proliferation was detected by MTT. Results （ 1 ） Rosiglitazone was able to inhibit NIT-lcells apoptosis caused by high concentration of glucose, after 48 hours＇ treatment with rosiglitazone （ 10^-5 mmol/L） ,cells apoptosis caused by glucose with concentration of 16.7 mmol/L,22.5 mmol/L and 27.6 mmot/L was significantly inhibited. （2） Rosiglitazone was able to enhance NIT^-1 cells proliferation. （3） Rosiglitazone was able to improve NIT^-1 cells insulin secretion in different glucose concentration. Conclusion Rosiglitazone can improve the islet B cells proliferation and function as well as inhibit apoptosis through direct effect on the islet B cells.