中文摘要：

通过还原反应合成纳米多孔Pt材料,并对其进行表征.纳米多孔Pt作为一种纳米模拟酶,对过氧化氢氧化3,3＇,5,5＇-四甲基联苯胺（TMB）形成蓝色氧化产物的反应具有良好的催化作用.加入多巴胺（DA）后,由于DA吸附到纳米多孔Pt的孔道上,减少了催化位点,对纳米多孔Pt的催化作用产生抑制效应,使形成的TMB蓝色氧化产物减少,导致吸光度值下降.研究利用多巴胺对纳米多孔Pt催化作用的抑制效应,采用分光光度计检测体系的吸光度从而实现多巴胺的定量检测.考察了过氧化氢浓度,多孔Pt浓度以及多巴胺反应时间对吸光度的影响.在最优条件下,吸光度值与多巴胺浓度有良好的线性关系,线性范围为0.08~1.0 mmol/L,相关系数为0.995 9,检测下限为0.008 5 mmol/L.该体系为多巴胺的检测提供了一种简单快速的方法,并被用于黑色素瘤细胞裂解液样品中多巴胺的检测.

英文摘要：

In this paper, nano-porous Pt materials was synthesized by reduction reaction, and was character- ized by TEM.Dopamine can inhibit the catalytic activity of bio-enzyme.Nano-porous Pt materials, as a nano- mimic enzyme ,was inhibited by dopamin as well.Porous Pt NPs have a good catalytic activity on the reduction of H202, and the above system combined with TMB will have a chromogenic reaction.In this study,inhibitory effect of dopamine to the catalysis of porous Pt NPs was used to realize the quantitative detection of dopamine using ul- traviolet spectrophotometer （UV-Vis） to detect the absorbance of the system.The concentration of H202 ,porous Pt NPs and the reacting time between porous Pt NPs and DA were optimized.Under the optimal experimental con- ditions, the detection range of the system is from 0.08 mmol/L to 1.0 mmol/L with a good linear correlation coef- ficient of 0.9959 and the detection limit of 0.008 5 mmol/L.This system provides a new,simple as well rapid method on the detection of DA and can be used for the detection of DA in cell.